Possible Association between Cathepsin v and the Development of Placenta Accreta Spectrum Disorders

Satoshi Matsukawa, Seiji Sumigama, Tomomi Kotani, Jingwen Wang, Rika Miki, Yoshinori Moriyama, Tomoko Nakano, Yukio Mano, Hiroyuki Tsuda, Koji Tamakoshi, Fumitaka Kikkawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Background/Aims: The study aimed to evaluate molecular changes related to trophoblast adhesion in placenta accreta spectrum (PAS) disorders. Methods: A retrospective analysis of 10 PAS cases in which both the trophoblast adherent site and the non-adherent site were identified was performed in April 2010 and March 2013. Microarray analysis and reverse transcription polymerase chain reaction (RT-PCR) analyses were performed to extract upregulated genes in the adherent site. Gene expression changes were examined by immunohistochemistry. Results: Microarray analysis showed that 157 transcripts were > 3-fold upregulated, including the following: a disintegrin and metalloproteinase-28 (ADAM28), 3.10-fold; cathepsin V (CTSV), 3.73-fold; cathepsin S (CTSS), 3.46-fold; and matrix metalloproteinase-19 (MMP19), 3.41-fold. RT-PCR showed relatively high mRNA expressions. On immunohistochemistry, extravillous trophoblast (EVT) at the non-adherent site showed weak or no CTSV expression, whereas EVT that invaded myometrium at the adherent site showed strong expression (histological score, median [min-max], 115.6 [37.6-153.6] vs. 184.8 [56.4-222.8], p < 0.05). MMP19 showed moderate staining, with no difference between the adherent and non-adherent sites. ADAM28 and CTSS showed weak or no staining. Discussion: This limited study suggests that CTSV may be involved in the pathogenesis of PAS.

Original languageEnglish
Pages (from-to)396-406
Number of pages11
JournalGynecologic and Obstetric Investigation
Issue number4
Publication statusPublished - 01-07-2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology


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