TY - JOUR
T1 - Possible involvement of catalase in the protective effect of interleukin-6 against 6-hydroxydopamine toxicity in pc12 cells
AU - Yamada, Kiyofumi
AU - Umegaki, Hiroyuki
AU - Maezawa, Izumi
AU - Iguchi, Akihisa
AU - Kameyama, Tsutomu
AU - Nabeshima, Toshitaka
N1 - Funding Information:
This study was supported in part by grants from the Ministry of Education, Science and Culture (No. 07557009 and 07557303), SRF Grant for Biomedical Research, and the Japan Research Foundation for Clinical Pharmacology.
PY - 1997
Y1 - 1997
N2 - We examined the effects of various neurotrophic factors and cytokines on 6-hydroxydopamine (6-OHDA)-induced toxicity in PC12 cells. Exposure of PC12 cells to 6-OHDA resulted in a concentration- and time-dependent cell death, as evidenced by the release of lactate dehydrogenase into the culture medium. Addition of catalase, but not superoxide dismutase, to the culture medium protected PC12 cells from the 6-OHDA-induced toxicity. Interleukin (IL)-6 provided a dose-dependent protection against the 6-OHDA toxicity, as did nerve growth factor (NGF). In addition, basic fibroblast growth factor and dibutyryl cyclic AMP partially protected PC12 cells from 6-OHDA toxicity. Neither IL-1α, IL-2, IL-4, transforming growth factor-β, nor leukemia inhibitory factor had any effect. The protective effect of IL-6 was attenuated by 3-amino-1,2,4-triazole, an inhibitor of catalase. These results suggest that IL-6 may protect PC12 cells against the 6-OHDA toxicity by activating free radical detoxifying mechanisms, such as catalase activity.
AB - We examined the effects of various neurotrophic factors and cytokines on 6-hydroxydopamine (6-OHDA)-induced toxicity in PC12 cells. Exposure of PC12 cells to 6-OHDA resulted in a concentration- and time-dependent cell death, as evidenced by the release of lactate dehydrogenase into the culture medium. Addition of catalase, but not superoxide dismutase, to the culture medium protected PC12 cells from the 6-OHDA-induced toxicity. Interleukin (IL)-6 provided a dose-dependent protection against the 6-OHDA toxicity, as did nerve growth factor (NGF). In addition, basic fibroblast growth factor and dibutyryl cyclic AMP partially protected PC12 cells from 6-OHDA toxicity. Neither IL-1α, IL-2, IL-4, transforming growth factor-β, nor leukemia inhibitory factor had any effect. The protective effect of IL-6 was attenuated by 3-amino-1,2,4-triazole, an inhibitor of catalase. These results suggest that IL-6 may protect PC12 cells against the 6-OHDA toxicity by activating free radical detoxifying mechanisms, such as catalase activity.
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U2 - 10.1016/S0361-9230(96)00336-X
DO - 10.1016/S0361-9230(96)00336-X
M3 - Article
C2 - 9254029
AN - SCOPUS:0030744643
SN - 0361-9230
VL - 43
SP - 573
EP - 577
JO - Brain Research Bulletin
JF - Brain Research Bulletin
IS - 6
ER -