Possible involvement of catalase in the protective effect of interleukin-6 against 6-hydroxydopamine toxicity in pc12 cells

Kiyofumi Yamada, Hiroyuki Umegaki, Izumi Maezawa, Akihisa Iguchi, Tsutomu Kameyama, Toshitaka Nabeshima

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We examined the effects of various neurotrophic factors and cytokines on 6-hydroxydopamine (6-OHDA)-induced toxicity in PC12 cells. Exposure of PC12 cells to 6-OHDA resulted in a concentration- and time-dependent cell death, as evidenced by the release of lactate dehydrogenase into the culture medium. Addition of catalase, but not superoxide dismutase, to the culture medium protected PC12 cells from the 6-OHDA-induced toxicity. Interleukin (IL)-6 provided a dose-dependent protection against the 6-OHDA toxicity, as did nerve growth factor (NGF). In addition, basic fibroblast growth factor and dibutyryl cyclic AMP partially protected PC12 cells from 6-OHDA toxicity. Neither IL-1α, IL-2, IL-4, transforming growth factor-β, nor leukemia inhibitory factor had any effect. The protective effect of IL-6 was attenuated by 3-amino-1,2,4-triazole, an inhibitor of catalase. These results suggest that IL-6 may protect PC12 cells against the 6-OHDA toxicity by activating free radical detoxifying mechanisms, such as catalase activity.

Original languageEnglish
Pages (from-to)573-577
Number of pages5
JournalBrain Research Bulletin
Volume43
Issue number6
DOIs
Publication statusPublished - 12-08-1997
Externally publishedYes

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Oxidopamine
PC12 Cells
Catalase
Interleukin-6
Culture Media
Amitrole
Leukemia Inhibitory Factor
Bucladesine
Nerve Growth Factors
Transforming Growth Factors
Nerve Growth Factor
Fibroblast Growth Factor 2
Interleukin-1
L-Lactate Dehydrogenase
Interleukin-4
Superoxide Dismutase
Free Radicals
Interleukin-2
Cell Death
Cytokines

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Yamada, Kiyofumi ; Umegaki, Hiroyuki ; Maezawa, Izumi ; Iguchi, Akihisa ; Kameyama, Tsutomu ; Nabeshima, Toshitaka. / Possible involvement of catalase in the protective effect of interleukin-6 against 6-hydroxydopamine toxicity in pc12 cells. In: Brain Research Bulletin. 1997 ; Vol. 43, No. 6. pp. 573-577.
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Possible involvement of catalase in the protective effect of interleukin-6 against 6-hydroxydopamine toxicity in pc12 cells. / Yamada, Kiyofumi; Umegaki, Hiroyuki; Maezawa, Izumi; Iguchi, Akihisa; Kameyama, Tsutomu; Nabeshima, Toshitaka.

In: Brain Research Bulletin, Vol. 43, No. 6, 12.08.1997, p. 573-577.

Research output: Contribution to journalArticle

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AU - Kameyama, Tsutomu

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