Possible involvement of p38 MAP kinase in prostaglandin E1-induced ALP activity in osteoblast-like cells

Ayako Kakita, Atsushi Suzuki, Yasunaga Ono, Yoshitaka Miura, Mitsuyasu Itoh, Yutaka Oiso

Research output: Contribution to journalArticle

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Abstract

Prostaglandins are now recognized to be important regulators for both bone formation and resorption. Among them, prostaglandin E 1 (PGE 1 ) has been reported to stimulate cAMP accumulation and to induce alkaline phosphatase (ALP) activity, a marker of differentiation, in osteoblast-like cells. Recently, we have shown that p38 mitogen-activated protein (MAP) kinase pathway regulates ALP activity in response to activation of Gi protein-coupled receptors in mouse osteoblast-like MC3T3-E1 cells (Suzuki et al., Endocrinology 140 (1999) 3177). In the present study, we investigated whether p38 MAP kinase is involved in ALP activation by PGE 1 in MC3T3-E1 osteoblast-like cells. PGE 1 dose-dependently enhanced ALP activities in the concentration range between 1 nM and 1 μM in MC3T3-E1 cells. SB203580, a specific inhibitor of p38 MAP kinase, blocked the increase in ALP activity induced by PGE 1 . Further analysis with western blotting suggested that PGE 1 induced an increase in tyrosine (Tyr) phosphorylation of p38 MAP kinase. Both Bt 2 CAMP, a permeable analogue of cAMP, and forskolin, which directly activates adenylate cyclase, also induced an increase in Tyr phosphorylation of p38 MAP kinase. H-89, a potent inhibitor of protein kinase A (PKA), significantly suppressed PGE 1 -induced Tyr phosphorylation of p38 MAP kinase. The results of this study suggest that PGE 1 stimulates p38 MAP kinase through the activation of PKA, resulting in the enhancement of ALP activity.

Original languageEnglish
Pages (from-to)469-474
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume70
Issue number5
DOIs
Publication statusPublished - 01-01-2004

Fingerprint

Alprostadil
Osteoblasts
p38 Mitogen-Activated Protein Kinases
Prostaglandins E
Alkaline Phosphatase
Phosphorylation
Tyrosine
Chemical activation
Cyclic AMP-Dependent Protein Kinases
Endocrinology
Differentiation Antigens
Colforsin
Bone Resorption
Adenylyl Cyclases
Osteogenesis
Prostaglandins
Bone
Western Blotting
Proteins

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

Cite this

Kakita, Ayako ; Suzuki, Atsushi ; Ono, Yasunaga ; Miura, Yoshitaka ; Itoh, Mitsuyasu ; Oiso, Yutaka. / Possible involvement of p38 MAP kinase in prostaglandin E1-induced ALP activity in osteoblast-like cells. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 2004 ; Vol. 70, No. 5. pp. 469-474.
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Possible involvement of p38 MAP kinase in prostaglandin E1-induced ALP activity in osteoblast-like cells. / Kakita, Ayako; Suzuki, Atsushi; Ono, Yasunaga; Miura, Yoshitaka; Itoh, Mitsuyasu; Oiso, Yutaka.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 70, No. 5, 01.01.2004, p. 469-474.

Research output: Contribution to journalArticle

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T1 - Possible involvement of p38 MAP kinase in prostaglandin E1-induced ALP activity in osteoblast-like cells

AU - Kakita, Ayako

AU - Suzuki, Atsushi

AU - Ono, Yasunaga

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AU - Itoh, Mitsuyasu

AU - Oiso, Yutaka

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