Possible involvement of Rab11 p24, a Ras-like small GTP-binding protein, in intracellular vesicular transport of isolated pancreatic acini

Yuichi Hori, Yoshifumi Takeyama, Motoki Hiroyoshi, Takashi Ueda, Akio Maeda, Harumasa Ohyanagi, Yoichi Saitoh, Kozo Kaibuchi, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Rab11 p24 is a Ras-like small guanosine triphosphate (GTP)-binding protein, and specific antibodies against it were newly developed to explore its function. Using the antibody, Rab11 p24 was shown to be abundant in rat pancreas as well as in most rat tissues. To explore the involvement of Rab11 p24 into the exocytotic process, the subcellular distribution of Rab11 p24 in rat pancreatic acini was evaluated also by use of the antibody. When the isolated acini were incubated with 1 x 10-10 M cholecystokinin octapeptide (CCK-8) that induced the maximal stimulation, the amount of Rab11 p24 increased in the fractions of plasma membrane and zymogen granules, but decreased in the cytosol fraction. This redistribution was time-dependent and occurred within 1 min after the CCK-8 stimulation and reached a maximal level within 2 min after the stimulation. Moreover, a light microscopic immunolabeling technique on the isolated rat pancreatic acini also revealed that higher immunoreactivity with Rab11 p24 was observed over the zymogen granule membrane under CCK-8 stimulation. The present results indicate that Rab11 p24 is translocated from cytosol to the membrane fraction during stimulation with CCK-8 and suggest that Rab11 p24 is involved in the intracellular vesicular transport of isolated pancreatic acini.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalDigestive Diseases and Sciences
Volume41
Issue number1
DOIs
Publication statusPublished - 1996

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

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