TY - JOUR
T1 - Possible therapeutic option of aqueous plasma for refractory ovarian cancer
AU - Kajiyama, Hiroaki
AU - Utsumi, Fumi
AU - Nakamura, Kae
AU - Tanaka, Hiromasa
AU - Mizuno, Masaaki
AU - Toyokuni, Shinya
AU - Hori, Masaru
AU - Kikkawa, Fumitaka
N1 - Publisher Copyright:
© 2015 Elsevier GmbH
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Based on a number of recent reports, plasma exerts anti-proliferative and apoptotic effects on various cancer cells. Besides a direct effect on the cells, the proliferation of normal and tumorous mammalian cells was reported to be downregulated by indirect plasma-exposed medium through the generation of reactive oxidative species. We demonstrated that plasma-activated medium (PAM) also had an anti-tumor effect on even acquired chemoresistant OC cells. Furthermore, the aqueous plasma displayed an anti-tumor effect against clear-cell carcinoma, which is natively chemo-refractory OC, and we confirmed that the efficacy is selective for tumor cells rather than normal mesothelial cells. The adhesion potency to type-I-collagen-coated dishes was significantly lower in both chemosensitive and chemoresistant cells pretreated with PAM than that of non-stimulated cells (P<0.0001). In particular, the adhesion potential of acquired chemoresistant cells was more evident than that of the original chemosensitive cells. Taken together, PAM reduced not only the proliferative activity but also attachment to extracellular matrix components. Here, a possible application of this technologic modality as a therapeutic target for OC is proposed.
AB - Based on a number of recent reports, plasma exerts anti-proliferative and apoptotic effects on various cancer cells. Besides a direct effect on the cells, the proliferation of normal and tumorous mammalian cells was reported to be downregulated by indirect plasma-exposed medium through the generation of reactive oxidative species. We demonstrated that plasma-activated medium (PAM) also had an anti-tumor effect on even acquired chemoresistant OC cells. Furthermore, the aqueous plasma displayed an anti-tumor effect against clear-cell carcinoma, which is natively chemo-refractory OC, and we confirmed that the efficacy is selective for tumor cells rather than normal mesothelial cells. The adhesion potency to type-I-collagen-coated dishes was significantly lower in both chemosensitive and chemoresistant cells pretreated with PAM than that of non-stimulated cells (P<0.0001). In particular, the adhesion potential of acquired chemoresistant cells was more evident than that of the original chemosensitive cells. Taken together, PAM reduced not only the proliferative activity but also attachment to extracellular matrix components. Here, a possible application of this technologic modality as a therapeutic target for OC is proposed.
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U2 - 10.1016/j.cpme.2015.12.002
DO - 10.1016/j.cpme.2015.12.002
M3 - Article
AN - SCOPUS:84952908385
SN - 2212-8166
VL - 4
SP - 14
EP - 18
JO - Clinical Plasma Medicine
JF - Clinical Plasma Medicine
IS - 1
ER -