TY - JOUR
T1 - Postnatal development of rat vocal folds
AU - Tateya, Tomoko
AU - Tateya, Ichiro
AU - Munoz-Del-Rio, Alejandro
AU - Bless, Diane M.
N1 - Funding Information:
From the Division of Otolaryngology–Head and Neck Surgery, University of Wisconsin–Madison, Madison, Wisconsin. This study was supported by grant R01 DC4428 from the National Institute on Deafness and Other Communication Disorders. This study was performed in accordance with the PHS Policy on Humane Care and Use of Laboratory Animals, the NIH Guide for the Care and Use of Laboratory Animals, and the Animal Welfare Act (7 U.S.C. et seq.); the animal use protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Wisconsin–Madison.
PY - 2006/3
Y1 - 2006/3
N2 - Objectives: In this study we aimed to determine the feasibility of using a rat model for the study of postnatal vocal fold (VF) development. Methods: Eighteen male rats that were 3 days old, 3 weeks old, or 8 months old were analyzed histologically with Alcian blue stain used for detecting hyaluronic acid, elastin-van Gieson stain for elastin, Oil Red O and gold chloride stains for vitamin A-containing lipid droplets, and immunohistochemistry for vimentin (general fibroblast marker) and collagen types I and III. Results: The macula flava (MF) was observed as a mass of cells that expressed vimentin intensively in the cytoplasm. The MF showed denser hyaluronic acid and collagen type I than did the midmembranous portion of the VF lamina propria. Clear developmental changes were evident in the MF and other regions. The vimentin-positive cells of the 3-day-old MF were mainly oval-shaped and had less cytoplasm, whereas those of the 8-month-old MF were spindle- and stellate-shaped and had more cytoplasm, similar to that reported in humans. Vitamin A-containing lipid droplets were limited to the 3-week-old and 8-month-old MFs and were not present in the 3-day-old VF. Conclusions: These results suggest that a rat model is useful in studying VF development and that vitamin A is related to the maturity of the VF.
AB - Objectives: In this study we aimed to determine the feasibility of using a rat model for the study of postnatal vocal fold (VF) development. Methods: Eighteen male rats that were 3 days old, 3 weeks old, or 8 months old were analyzed histologically with Alcian blue stain used for detecting hyaluronic acid, elastin-van Gieson stain for elastin, Oil Red O and gold chloride stains for vitamin A-containing lipid droplets, and immunohistochemistry for vimentin (general fibroblast marker) and collagen types I and III. Results: The macula flava (MF) was observed as a mass of cells that expressed vimentin intensively in the cytoplasm. The MF showed denser hyaluronic acid and collagen type I than did the midmembranous portion of the VF lamina propria. Clear developmental changes were evident in the MF and other regions. The vimentin-positive cells of the 3-day-old MF were mainly oval-shaped and had less cytoplasm, whereas those of the 8-month-old MF were spindle- and stellate-shaped and had more cytoplasm, similar to that reported in humans. Vitamin A-containing lipid droplets were limited to the 3-week-old and 8-month-old MFs and were not present in the 3-day-old VF. Conclusions: These results suggest that a rat model is useful in studying VF development and that vitamin A is related to the maturity of the VF.
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U2 - 10.1177/000348940611500310
DO - 10.1177/000348940611500310
M3 - Article
C2 - 16572612
AN - SCOPUS:33644923477
VL - 115
SP - 215
EP - 224
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
SN - 0003-4894
IS - 3
ER -