TY - JOUR
T1 - Potential limitations in using minor histocompatibility antigen-specific cytotoxic T cells for targeting solid tumor cells
AU - Miyazaki, Mikinori
AU - Akatsuka, Yoshiki
AU - Nishida, Tetsuya
AU - Fujii, Nobuharu
AU - Hiraki, Akio
AU - Ikeda, Kazuma
AU - Tsujimura, Kunio
AU - Kuzushima, Kiyotaka
AU - Morishima, Yasuo
AU - Sato, Shigeki
AU - Ueda, Ryuzo
AU - Takahashi, Toshitada
N1 - Funding Information:
This work was supported in part by Grant-in-Aid for Scientific Research (C) (Y.A., T.T.) and Scientific Research on Priority Areas (T.T., K.T., Y.A.), from the Ministry of Education, Culture, Science, Sports, and Technology, Japan; Research on Human Genome, Tissue Engineering Food Biotechnology (Y.A.); Second Term Comprehensive 10-year Strategy for Cancer Control from the Ministry of Health, Labour, and Welfare, Japan (T.T.); and Nagono Medical Research Grant (K.K.).
PY - 2003/6/1
Y1 - 2003/6/1
N2 - We have shown previously that KIAA0223, a gene encoding a minor histocompatibility antigen, HA-1, whose expression was believed to be restricted to the hematopoietic cells, is aberrantly expressed in some solid tumor cell lines. However, its significance in tumor immunity needs to be determined. Cytotoxic activity of HA-1H-specific cytotoxic T lymphocytes (CTLs) was assessed against solid tumor cell lines expressing KIAA0223 using 51Cr release assays. Five of seven cell lines were lysed when HLA-A*0201 was adequately expressed. One of the two CTL-resistant cell lines became susceptible after treatment with IFN-γ and TNF-α, while the other was lysed only after pulsing with HA-1H peptide. In most cell lines tested, HA-1H peptide was properly generated and presented for recognition by the CTL. However, impaired antigen processing and presentation observed in this study may result in escape from CTL recognition in vivo, as well as in vitro, as observed in this study.
AB - We have shown previously that KIAA0223, a gene encoding a minor histocompatibility antigen, HA-1, whose expression was believed to be restricted to the hematopoietic cells, is aberrantly expressed in some solid tumor cell lines. However, its significance in tumor immunity needs to be determined. Cytotoxic activity of HA-1H-specific cytotoxic T lymphocytes (CTLs) was assessed against solid tumor cell lines expressing KIAA0223 using 51Cr release assays. Five of seven cell lines were lysed when HLA-A*0201 was adequately expressed. One of the two CTL-resistant cell lines became susceptible after treatment with IFN-γ and TNF-α, while the other was lysed only after pulsing with HA-1H peptide. In most cell lines tested, HA-1H peptide was properly generated and presented for recognition by the CTL. However, impaired antigen processing and presentation observed in this study may result in escape from CTL recognition in vivo, as well as in vitro, as observed in this study.
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U2 - 10.1016/S1521-6616(03)00065-2
DO - 10.1016/S1521-6616(03)00065-2
M3 - Article
C2 - 12804533
AN - SCOPUS:0038541695
SN - 1521-6616
VL - 107
SP - 198
EP - 201
JO - Clinical Immunology
JF - Clinical Immunology
IS - 3
ER -