TY - JOUR
T1 - Potential link between Fusobacterium enrichment and DNA methylation accumulation in the inflammatory colonic mucosa in ulcerative colitis
AU - Tahara, Tomomitsu
AU - Hirata, Ichiro
AU - Nakano, Naoko
AU - Tahara, Sayumi
AU - Horiguchi, Noriyuki
AU - Kawamura, Tomohiko
AU - Okubo, Masaaki
AU - Ishizuka, Takamitsu
AU - Yamada, Hyuga
AU - Yoshida, Dai
AU - Ohmori, Takafumi
AU - Maeda, Kohei
AU - Komura, Naruomi
AU - Ikuno, Hirokazu
AU - Jodai, Yasutaka
AU - Kamano, Toshiaki
AU - Nagasaka, Mitsuo
AU - Nakagawa, Yoshihito
AU - Tuskamoto, Tetsuya
AU - Urano, Makoto
AU - Shibata, Tomoyuki
AU - Kuroda, Makoto
AU - Ohmiya, Naoki
N1 - Funding Information:
This study was funded by the Uehara Memorial Foundation.
Publisher Copyright:
© Tahara et al.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - BACKGROUND AND AIM: Fusobacterium enrichment has been associated with colorectal cancer development. Ulcerative colitis (UC) associated tumorigenesis is characterized as high degree of methylation accumulation through continuous colonic inflammation. The aim of this study was to investigate a potential link between Fusobacterium enrichment and DNA methylation accumulation in the inflammatory colonic mucosa in UC. METHODS: In the candidate analysis, inflamed colonic mucosa from 86 UC patients were characterized the methylation status of colorectal a panel of cancer related 24 genes. In the genome-wide analysis, an Infinium HumanMethylation450 BeadChip array was utilized to characterize the methylation status of > 450,000 CpG sites for fourteen UC patients. Results were correlated with Fusobacterium status. RESULTS: UC with Fusobacterium enrichment (FB-high) was characterized as high degree of type C (for cancer-specific) methylation compared to other (FB-low/ neg) samples (P < 0.01). Genes hypermethylated in FB-high samples included wellknown type C genes in colorectal cancer, such as MINT2 and 31, P16 and NEUROG1. Multivariate analysis demonstrated that the FB high status held an increased likelihood for methylation high as an independent factor (odds ratio: 16.18, 95% confidence interval: 1.94-135.2, P=0.01). Genome-wide methylation analysis demonstrated a unique methylome signature of FB-high cases irrespective of promoter, outside promoter, CpG and non-CpG sites. Group of promoter CpG sites that were exclusively hypermethylated in FB-high cases significantly codified the genes related to the catalytic activity (P=0.039). CONCLUSION: Our findings suggest that Fusobacterium accelerates DNA methylation in specific groups of genes in the inflammatory colonic mucosa in UC.
AB - BACKGROUND AND AIM: Fusobacterium enrichment has been associated with colorectal cancer development. Ulcerative colitis (UC) associated tumorigenesis is characterized as high degree of methylation accumulation through continuous colonic inflammation. The aim of this study was to investigate a potential link between Fusobacterium enrichment and DNA methylation accumulation in the inflammatory colonic mucosa in UC. METHODS: In the candidate analysis, inflamed colonic mucosa from 86 UC patients were characterized the methylation status of colorectal a panel of cancer related 24 genes. In the genome-wide analysis, an Infinium HumanMethylation450 BeadChip array was utilized to characterize the methylation status of > 450,000 CpG sites for fourteen UC patients. Results were correlated with Fusobacterium status. RESULTS: UC with Fusobacterium enrichment (FB-high) was characterized as high degree of type C (for cancer-specific) methylation compared to other (FB-low/ neg) samples (P < 0.01). Genes hypermethylated in FB-high samples included wellknown type C genes in colorectal cancer, such as MINT2 and 31, P16 and NEUROG1. Multivariate analysis demonstrated that the FB high status held an increased likelihood for methylation high as an independent factor (odds ratio: 16.18, 95% confidence interval: 1.94-135.2, P=0.01). Genome-wide methylation analysis demonstrated a unique methylome signature of FB-high cases irrespective of promoter, outside promoter, CpG and non-CpG sites. Group of promoter CpG sites that were exclusively hypermethylated in FB-high cases significantly codified the genes related to the catalytic activity (P=0.039). CONCLUSION: Our findings suggest that Fusobacterium accelerates DNA methylation in specific groups of genes in the inflammatory colonic mucosa in UC.
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U2 - 10.18632/oncotarget.18716
DO - 10.18632/oncotarget.18716
M3 - Article
AN - SCOPUS:85028775713
VL - 8
SP - 61917
EP - 61926
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 37
ER -