Potential role for matrix metallo- proteinase-3 in gastric ulcer healing

Makoto Tomita, Takafumi Ando, Masaaki Minami, Osamu Watanabe, Kazuhiro Ishiguro, Motofusa Hasegawa, Nobuyuki Miyake, Shinya Kondo, Tsuyoshi Kato, Ryoji Miyahara, Naoki Omiya, Yasumasa Niwa, Hidemi Goto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background/Aims: Matrix metalloproteinases (MMPs) are endopeptidases which perform important functions in extracellular matrix remodeling, cell proliferation, and inflammatory processes. Here, we compared MMP-3 levels with those of tissue inhibitor of metalloproteinases (TIMP)-1 and several inflammatory cytokines in gastric ulcer (GU) patients. Methods: This study enrolled 50 patients with GU and 6 with functional dyspepsia (FD). Samples of gastric mucosa from the antrum and the ulcer site were harvested from GU patients and of antral mucosa alone from FD patients during upper gastrointestinal endoscopy. Mucosal biopsy tissues were cultured for 24 h, and the culture supernatant was measured for levels of MMP-3, TIMP-1, IL-1β, IL-6, and IL-8. Results: All GU patients were positive for Helicobacter pylori, while all FD patients were negative. Antral levels of TIMP-1, IL-1β, IL-6, and IL-8 were significantly higher in GU than FD patients. Further, MMP-3 levels were significantly higher in GU patients at the ulcer site than in the antrum, and had a significantly positive correlation with TIMP-1, IL-1β, IL-6, and IL-8. Conclusion: MMP-3 levels were significantly higher at the ulcer site than in the antrum, suggesting that MMP-3 may perform an important function in gastric ulcer healing.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalDigestion
Volume79
Issue number1
DOIs
Publication statusPublished - 01-03-2009
Externally publishedYes

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Myeloblastin
Stomach Ulcer
Matrix Metalloproteinase 3
Tissue Inhibitor of Metalloproteinase-1
Dyspepsia
Interleukin-8
Interleukin-1
Ulcer
Interleukin-6
Mucous Membrane
Pyloric Antrum
Endopeptidases
Gastrointestinal Endoscopy
Gastric Mucosa
Matrix Metalloproteinases
Helicobacter pylori
Extracellular Matrix
Cell Proliferation
Cytokines
Biopsy

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Tomita, M., Ando, T., Minami, M., Watanabe, O., Ishiguro, K., Hasegawa, M., ... Goto, H. (2009). Potential role for matrix metallo- proteinase-3 in gastric ulcer healing. Digestion, 79(1), 23-29. https://doi.org/10.1159/000203637
Tomita, Makoto ; Ando, Takafumi ; Minami, Masaaki ; Watanabe, Osamu ; Ishiguro, Kazuhiro ; Hasegawa, Motofusa ; Miyake, Nobuyuki ; Kondo, Shinya ; Kato, Tsuyoshi ; Miyahara, Ryoji ; Omiya, Naoki ; Niwa, Yasumasa ; Goto, Hidemi. / Potential role for matrix metallo- proteinase-3 in gastric ulcer healing. In: Digestion. 2009 ; Vol. 79, No. 1. pp. 23-29.
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Tomita, M, Ando, T, Minami, M, Watanabe, O, Ishiguro, K, Hasegawa, M, Miyake, N, Kondo, S, Kato, T, Miyahara, R, Omiya, N, Niwa, Y & Goto, H 2009, 'Potential role for matrix metallo- proteinase-3 in gastric ulcer healing', Digestion, vol. 79, no. 1, pp. 23-29. https://doi.org/10.1159/000203637

Potential role for matrix metallo- proteinase-3 in gastric ulcer healing. / Tomita, Makoto; Ando, Takafumi; Minami, Masaaki; Watanabe, Osamu; Ishiguro, Kazuhiro; Hasegawa, Motofusa; Miyake, Nobuyuki; Kondo, Shinya; Kato, Tsuyoshi; Miyahara, Ryoji; Omiya, Naoki; Niwa, Yasumasa; Goto, Hidemi.

In: Digestion, Vol. 79, No. 1, 01.03.2009, p. 23-29.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Potential role for matrix metallo- proteinase-3 in gastric ulcer healing

AU - Tomita, Makoto

AU - Ando, Takafumi

AU - Minami, Masaaki

AU - Watanabe, Osamu

AU - Ishiguro, Kazuhiro

AU - Hasegawa, Motofusa

AU - Miyake, Nobuyuki

AU - Kondo, Shinya

AU - Kato, Tsuyoshi

AU - Miyahara, Ryoji

AU - Omiya, Naoki

AU - Niwa, Yasumasa

AU - Goto, Hidemi

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Background/Aims: Matrix metalloproteinases (MMPs) are endopeptidases which perform important functions in extracellular matrix remodeling, cell proliferation, and inflammatory processes. Here, we compared MMP-3 levels with those of tissue inhibitor of metalloproteinases (TIMP)-1 and several inflammatory cytokines in gastric ulcer (GU) patients. Methods: This study enrolled 50 patients with GU and 6 with functional dyspepsia (FD). Samples of gastric mucosa from the antrum and the ulcer site were harvested from GU patients and of antral mucosa alone from FD patients during upper gastrointestinal endoscopy. Mucosal biopsy tissues were cultured for 24 h, and the culture supernatant was measured for levels of MMP-3, TIMP-1, IL-1β, IL-6, and IL-8. Results: All GU patients were positive for Helicobacter pylori, while all FD patients were negative. Antral levels of TIMP-1, IL-1β, IL-6, and IL-8 were significantly higher in GU than FD patients. Further, MMP-3 levels were significantly higher in GU patients at the ulcer site than in the antrum, and had a significantly positive correlation with TIMP-1, IL-1β, IL-6, and IL-8. Conclusion: MMP-3 levels were significantly higher at the ulcer site than in the antrum, suggesting that MMP-3 may perform an important function in gastric ulcer healing.

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Tomita M, Ando T, Minami M, Watanabe O, Ishiguro K, Hasegawa M et al. Potential role for matrix metallo- proteinase-3 in gastric ulcer healing. Digestion. 2009 Mar 1;79(1):23-29. https://doi.org/10.1159/000203637