Practical methods using boronic acid compounds for identification of class C β-lactamase-producing Klebsiella pneumoniae and Escherichia coli

Tetsuya Yagi, Jun Ichi Wachino, Hiroshi Kurokawa, Satowa Suzuki, Kunikazu Yamane, Yohei Doi, Naohiro Shibata, Haru Kato, Keigo Shibayama, Yoshichika Arakawa

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Detection of the resistance mediated by class C β-lactamases remains a challenging issue, considering that transferable plasmid-mediated class C β-lactamases are of worldwide concern. Methods for the identification of strains that produce extended-spectrum β-lactamases (ESBLs) or metallo-β-lactamases (MBLs) have been developed and applied for routine use in clinical microbiology laboratories, but no practical methods for identification of plasmid-mediated class C producers have been established to date. We therefore developed three simple methods for clinical microbiology laboratories that allow identification of plasmid-mediated class C β-lactamase-producing bacteria using a boronic acid derivative, 3-aminophenylboronic acid (APB), one of the specific inhibitors of class C β-lactamases. Detection by the disk potentiation test was based on the enlargement of the growth-inhibitory zone diameter (by greater than or equal to 5 mm) around a Kirby-Bauer disk containing a ceftazidime (CAZ) or a cefotaxime (CTX) disk in combination with APB. In a double-disk synergy test, the discernible expansion of the growth-inhibitory zone around the CAZ or the CTX disk toward a disk containing APB was indicative of class C β-lactamase production. A greater than or equal to eightfold decrease in the MIC of CAZ or CTX in the presence of APB was the criterion for detection in the microdilution test. By using these methods, Escherichia coli and Klebsiella pneumoniae isolates producing plasmid-mediated class C β-lactamases, ACT-1, CMY-2, CMY-9, FOX-5, LAT-1, and MOX-1, were successfully distinguished from those producing other classes of β-lactamases, such as ESBLs and MBLs. These methods will provide useful information needed for targeted antimicrobial therapy and better infection control.

Original languageEnglish
Pages (from-to)2551-2558
Number of pages8
JournalJournal of clinical microbiology
Volume43
Issue number6
DOIs
Publication statusPublished - 01-06-2005
Externally publishedYes

Fingerprint

Boronic Acids
Klebsiella pneumoniae
Ceftazidime
Cefotaxime
Escherichia coli
Plasmids
Microbiology
Infection Control
Growth
Bacteria
3-aminobenzeneboronic acid

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Yagi, Tetsuya ; Wachino, Jun Ichi ; Kurokawa, Hiroshi ; Suzuki, Satowa ; Yamane, Kunikazu ; Doi, Yohei ; Shibata, Naohiro ; Kato, Haru ; Shibayama, Keigo ; Arakawa, Yoshichika. / Practical methods using boronic acid compounds for identification of class C β-lactamase-producing Klebsiella pneumoniae and Escherichia coli. In: Journal of clinical microbiology. 2005 ; Vol. 43, No. 6. pp. 2551-2558.
@article{45b2cf5017d44e3eb61910c08fda3ade,
title = "Practical methods using boronic acid compounds for identification of class C β-lactamase-producing Klebsiella pneumoniae and Escherichia coli",
abstract = "Detection of the resistance mediated by class C β-lactamases remains a challenging issue, considering that transferable plasmid-mediated class C β-lactamases are of worldwide concern. Methods for the identification of strains that produce extended-spectrum β-lactamases (ESBLs) or metallo-β-lactamases (MBLs) have been developed and applied for routine use in clinical microbiology laboratories, but no practical methods for identification of plasmid-mediated class C producers have been established to date. We therefore developed three simple methods for clinical microbiology laboratories that allow identification of plasmid-mediated class C β-lactamase-producing bacteria using a boronic acid derivative, 3-aminophenylboronic acid (APB), one of the specific inhibitors of class C β-lactamases. Detection by the disk potentiation test was based on the enlargement of the growth-inhibitory zone diameter (by greater than or equal to 5 mm) around a Kirby-Bauer disk containing a ceftazidime (CAZ) or a cefotaxime (CTX) disk in combination with APB. In a double-disk synergy test, the discernible expansion of the growth-inhibitory zone around the CAZ or the CTX disk toward a disk containing APB was indicative of class C β-lactamase production. A greater than or equal to eightfold decrease in the MIC of CAZ or CTX in the presence of APB was the criterion for detection in the microdilution test. By using these methods, Escherichia coli and Klebsiella pneumoniae isolates producing plasmid-mediated class C β-lactamases, ACT-1, CMY-2, CMY-9, FOX-5, LAT-1, and MOX-1, were successfully distinguished from those producing other classes of β-lactamases, such as ESBLs and MBLs. These methods will provide useful information needed for targeted antimicrobial therapy and better infection control.",
author = "Tetsuya Yagi and Wachino, {Jun Ichi} and Hiroshi Kurokawa and Satowa Suzuki and Kunikazu Yamane and Yohei Doi and Naohiro Shibata and Haru Kato and Keigo Shibayama and Yoshichika Arakawa",
year = "2005",
month = "6",
day = "1",
doi = "10.1128/JCM.43.6.2551-2558.2005",
language = "English",
volume = "43",
pages = "2551--2558",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

Practical methods using boronic acid compounds for identification of class C β-lactamase-producing Klebsiella pneumoniae and Escherichia coli. / Yagi, Tetsuya; Wachino, Jun Ichi; Kurokawa, Hiroshi; Suzuki, Satowa; Yamane, Kunikazu; Doi, Yohei; Shibata, Naohiro; Kato, Haru; Shibayama, Keigo; Arakawa, Yoshichika.

In: Journal of clinical microbiology, Vol. 43, No. 6, 01.06.2005, p. 2551-2558.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Practical methods using boronic acid compounds for identification of class C β-lactamase-producing Klebsiella pneumoniae and Escherichia coli

AU - Yagi, Tetsuya

AU - Wachino, Jun Ichi

AU - Kurokawa, Hiroshi

AU - Suzuki, Satowa

AU - Yamane, Kunikazu

AU - Doi, Yohei

AU - Shibata, Naohiro

AU - Kato, Haru

AU - Shibayama, Keigo

AU - Arakawa, Yoshichika

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Detection of the resistance mediated by class C β-lactamases remains a challenging issue, considering that transferable plasmid-mediated class C β-lactamases are of worldwide concern. Methods for the identification of strains that produce extended-spectrum β-lactamases (ESBLs) or metallo-β-lactamases (MBLs) have been developed and applied for routine use in clinical microbiology laboratories, but no practical methods for identification of plasmid-mediated class C producers have been established to date. We therefore developed three simple methods for clinical microbiology laboratories that allow identification of plasmid-mediated class C β-lactamase-producing bacteria using a boronic acid derivative, 3-aminophenylboronic acid (APB), one of the specific inhibitors of class C β-lactamases. Detection by the disk potentiation test was based on the enlargement of the growth-inhibitory zone diameter (by greater than or equal to 5 mm) around a Kirby-Bauer disk containing a ceftazidime (CAZ) or a cefotaxime (CTX) disk in combination with APB. In a double-disk synergy test, the discernible expansion of the growth-inhibitory zone around the CAZ or the CTX disk toward a disk containing APB was indicative of class C β-lactamase production. A greater than or equal to eightfold decrease in the MIC of CAZ or CTX in the presence of APB was the criterion for detection in the microdilution test. By using these methods, Escherichia coli and Klebsiella pneumoniae isolates producing plasmid-mediated class C β-lactamases, ACT-1, CMY-2, CMY-9, FOX-5, LAT-1, and MOX-1, were successfully distinguished from those producing other classes of β-lactamases, such as ESBLs and MBLs. These methods will provide useful information needed for targeted antimicrobial therapy and better infection control.

AB - Detection of the resistance mediated by class C β-lactamases remains a challenging issue, considering that transferable plasmid-mediated class C β-lactamases are of worldwide concern. Methods for the identification of strains that produce extended-spectrum β-lactamases (ESBLs) or metallo-β-lactamases (MBLs) have been developed and applied for routine use in clinical microbiology laboratories, but no practical methods for identification of plasmid-mediated class C producers have been established to date. We therefore developed three simple methods for clinical microbiology laboratories that allow identification of plasmid-mediated class C β-lactamase-producing bacteria using a boronic acid derivative, 3-aminophenylboronic acid (APB), one of the specific inhibitors of class C β-lactamases. Detection by the disk potentiation test was based on the enlargement of the growth-inhibitory zone diameter (by greater than or equal to 5 mm) around a Kirby-Bauer disk containing a ceftazidime (CAZ) or a cefotaxime (CTX) disk in combination with APB. In a double-disk synergy test, the discernible expansion of the growth-inhibitory zone around the CAZ or the CTX disk toward a disk containing APB was indicative of class C β-lactamase production. A greater than or equal to eightfold decrease in the MIC of CAZ or CTX in the presence of APB was the criterion for detection in the microdilution test. By using these methods, Escherichia coli and Klebsiella pneumoniae isolates producing plasmid-mediated class C β-lactamases, ACT-1, CMY-2, CMY-9, FOX-5, LAT-1, and MOX-1, were successfully distinguished from those producing other classes of β-lactamases, such as ESBLs and MBLs. These methods will provide useful information needed for targeted antimicrobial therapy and better infection control.

UR - http://www.scopus.com/inward/record.url?scp=20444481360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444481360&partnerID=8YFLogxK

U2 - 10.1128/JCM.43.6.2551-2558.2005

DO - 10.1128/JCM.43.6.2551-2558.2005

M3 - Article

C2 - 15956362

AN - SCOPUS:20444481360

VL - 43

SP - 2551

EP - 2558

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -