Pre-administration of L-tryptophan improved ADR-induced early renal failure in mice

Yuko Arioka, Yasuko Yamamoto, Masato Hoshi, Keishi Matsumoto, Manabu Takamatsu, Akira Hara, Mitsuru Seishima, Kuniaki Saito

Research output: Contribution to journalArticle

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Abstract

Aims: The aim of this study was to determine the localization of the rate-limiting enzyme indoleamine 2, 3-dioxygenase (IDO) and its metabolite in mice kidneys after adriamycin (ADR)-induced renal failure. We also examined the effect of l-tryptophan (Trp) administration on this model. Main methods: BALB/c mice were treated with 15 mg/kg ADR to induce renal failure. The change of IDO and l-kynurenine (Kyn) following ADR-induced renal failure was examined by immunostaining combined with hematoxylin and eosin (HE) and Periodic acid-Schiff (PAS) staining for morphological analysis, and the concentration of l-Kyn was measured by HPLC. The effect of l-Trp administration on ADR-induced renal failure was also investigated. Key findings: Time-dependent increase of IDO immunostaining was observed in tubular cells from Day 4 after ADR injection. It was found that mice fed with l-Trp had less chance of renal failure at four days after ADR injection than mice untreated with l-Trp, but not at seven days. Further, l-Trp treatment significantly suppressed an increased level of TNF-alpha mRNA, but not of TGF-beta and IL1-beta after renal injury. Significance: Local IDO expression in tubules was induced markedly after ADR- induced renal failure. l-Trp administration can be effective in suppressing ADR-induced renal failure at an early stage.

Original languageEnglish
Pages (from-to)100-106
Number of pages7
JournalLife Sciences
Volume91
Issue number3-4
DOIs
Publication statusPublished - 21-08-2012
Externally publishedYes

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Tryptophan
Doxorubicin
Renal Insufficiency
Indoleamine-Pyrrole 2,3,-Dioxygenase
Kynurenine
Kidney
Injections
Periodic Acid
Hematoxylin
Eosine Yellowish-(YS)
Metabolites
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
High Pressure Liquid Chromatography
Staining and Labeling
Messenger RNA
Wounds and Injuries
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Arioka, Yuko ; Yamamoto, Yasuko ; Hoshi, Masato ; Matsumoto, Keishi ; Takamatsu, Manabu ; Hara, Akira ; Seishima, Mitsuru ; Saito, Kuniaki. / Pre-administration of L-tryptophan improved ADR-induced early renal failure in mice. In: Life Sciences. 2012 ; Vol. 91, No. 3-4. pp. 100-106.
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Pre-administration of L-tryptophan improved ADR-induced early renal failure in mice. / Arioka, Yuko; Yamamoto, Yasuko; Hoshi, Masato; Matsumoto, Keishi; Takamatsu, Manabu; Hara, Akira; Seishima, Mitsuru; Saito, Kuniaki.

In: Life Sciences, Vol. 91, No. 3-4, 21.08.2012, p. 100-106.

Research output: Contribution to journalArticle

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T1 - Pre-administration of L-tryptophan improved ADR-induced early renal failure in mice

AU - Arioka, Yuko

AU - Yamamoto, Yasuko

AU - Hoshi, Masato

AU - Matsumoto, Keishi

AU - Takamatsu, Manabu

AU - Hara, Akira

AU - Seishima, Mitsuru

AU - Saito, Kuniaki

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