TY - JOUR
T1 - Predicted expansion of the claudin multigene family
AU - Mineta, Katsuhiko
AU - Yamamoto, Yasuko
AU - Yamazaki, Yuji
AU - Tanaka, Hiroo
AU - Tada, Yukiyo
AU - Saito, Kuniaki
AU - Tamura, Atsushi
AU - Igarashi, Michihiro
AU - Endo, Toshinori
AU - Takeuchi, Kosei
AU - Tsukita, Sachiko
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Creative Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), to Sachiko Tsukita, and by a Grants-in-Aid for Scientific Research from MEXT and for Research and Development Program for New Bio-industry Initiatives, to Kosei Takeuchi.
PY - 2011/2/18
Y1 - 2011/2/18
N2 - Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)
AB - Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)
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U2 - 10.1016/j.febslet.2011.01.028
DO - 10.1016/j.febslet.2011.01.028
M3 - Article
C2 - 21276448
AN - SCOPUS:79951575639
VL - 585
SP - 606
EP - 612
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 4
ER -