Predicted expansion of the claudin multigene family

Katsuhiko Mineta, Yasuko Yamamoto, Yuji Yamazaki, Hiroo Tanaka, Yukiyo Tada, Kuniaki Saito, Atsushi Tamura, Michihiro Igarashi, Toshinori Endo, Kosei Takeuchi, Sachiko Tsukita

Research output: Contribution to journalArticle

279 Citations (Scopus)

Abstract

Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)

Original languageEnglish
Pages (from-to)606-612
Number of pages7
JournalFEBS Letters
Volume585
Issue number4
DOIs
Publication statusPublished - 18-02-2011

Fingerprint

Claudins
Multigene Family
Tight Junctions
Fluorescence microscopy
Fluorescence Microscopy
Claudin-1
Membrane Proteins
Genes
Epithelial Cells
Tissue
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Mineta, K., Yamamoto, Y., Yamazaki, Y., Tanaka, H., Tada, Y., Saito, K., ... Tsukita, S. (2011). Predicted expansion of the claudin multigene family. FEBS Letters, 585(4), 606-612. https://doi.org/10.1016/j.febslet.2011.01.028
Mineta, Katsuhiko ; Yamamoto, Yasuko ; Yamazaki, Yuji ; Tanaka, Hiroo ; Tada, Yukiyo ; Saito, Kuniaki ; Tamura, Atsushi ; Igarashi, Michihiro ; Endo, Toshinori ; Takeuchi, Kosei ; Tsukita, Sachiko. / Predicted expansion of the claudin multigene family. In: FEBS Letters. 2011 ; Vol. 585, No. 4. pp. 606-612.
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Mineta, K, Yamamoto, Y, Yamazaki, Y, Tanaka, H, Tada, Y, Saito, K, Tamura, A, Igarashi, M, Endo, T, Takeuchi, K & Tsukita, S 2011, 'Predicted expansion of the claudin multigene family', FEBS Letters, vol. 585, no. 4, pp. 606-612. https://doi.org/10.1016/j.febslet.2011.01.028

Predicted expansion of the claudin multigene family. / Mineta, Katsuhiko; Yamamoto, Yasuko; Yamazaki, Yuji; Tanaka, Hiroo; Tada, Yukiyo; Saito, Kuniaki; Tamura, Atsushi; Igarashi, Michihiro; Endo, Toshinori; Takeuchi, Kosei; Tsukita, Sachiko.

In: FEBS Letters, Vol. 585, No. 4, 18.02.2011, p. 606-612.

Research output: Contribution to journalArticle

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AU - Tanaka, Hiroo

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AU - Saito, Kuniaki

AU - Tamura, Atsushi

AU - Igarashi, Michihiro

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AU - Takeuchi, Kosei

AU - Tsukita, Sachiko

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