Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum

Nipawan Nuemket, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, Isao Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Botulinum toxin (BoNT) from Clostridium botulinum OFD05, isolated from bovine botulism, is a D/C mosaic-type BoNT. BoNTs possess binding, translocation and catalytic domains. The BoNT/OFD05 binding domain exhibits significant sequence identity to BoNT/C, which requires a single ganglioside as a binding receptor on neuronal cells, while BoNT/A and BoNT/B require two receptors for specific binding. To determine the binding mechanism of BoNT/OFD05 and its ganglioside receptors on neuronal cells, recombinant BoNT/OFD05 receptor-binding domain has been expressed, purified and crystallized. Native and SeMet-derivative crystals showed X-ray diffraction to 2.8 and 3.1 Å resolution, respectively. The crystals belonged to space group P212121.

Original languageEnglish
Pages (from-to)608-610
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number5
DOIs
Publication statusPublished - 29-04-2010

Fingerprint

Clostridium botulinum
Dilatation and Curettage
Clostridium
Botulinum Toxins
Neurotoxins
X-Rays
X rays
x rays
Botulism
Crystals
Type A Botulinum Toxins
Gangliosides
cells
X-Ray Diffraction
crystals
Catalytic Domain
Derivatives
X ray diffraction

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Nuemket, Nipawan ; Tanaka, Yoshikazu ; Tsukamoto, Kentaro ; Tsuji, Takao ; Nakamura, Keiji ; Kozaki, Shunji ; Yao, Min ; Tanaka, Isao. / Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2010 ; Vol. 66, No. 5. pp. 608-610.
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Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum. / Nuemket, Nipawan; Tanaka, Yoshikazu; Tsukamoto, Kentaro; Tsuji, Takao; Nakamura, Keiji; Kozaki, Shunji; Yao, Min; Tanaka, Isao.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 5, 29.04.2010, p. 608-610.

Research output: Contribution to journalArticle

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AU - Nuemket, Nipawan

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AU - Tsuji, Takao

AU - Nakamura, Keiji

AU - Kozaki, Shunji

AU - Yao, Min

AU - Tanaka, Isao

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