TY - JOUR
T1 - Prenatal vitamin A deficiency causes laryngeal malformation in rats
AU - Tateya, Ichiro
AU - Tateya, Tomoko
AU - Surles, Rebecca Lynn
AU - Tanumihardjo, Sherry
AU - Bless, Diane M.
PY - 2007/10
Y1 - 2007/10
N2 - Objectives: Our previous research demonstrated that vitamin A might be related to vocal fold development. The purpose of this study was to determine whether vitamin A deficiency affects prenatal laryngeal development in rats. Methods: Two considerations were necessary in designing a study using a rat model: for embryonic survival, vitamin A is necessary through day 10 of gestation, and laryngeal formation occurs primarily after day 11. Thus, we created a rat model that developed vitamin A deficiency after embryonic day 11. Ten pregnant rats (5 vitamin A-deficient rats and 5 control rats) were studied. Embryos were collected at embryonic day 18.5 and analyzed histologically. Results: Eighteen percent of the vitamin A-deficient embryos were alive and demonstrated laryngotracheal cartilage malformation, incomplete separation of the glottis, and/or laryngoesophageal clefts. Conclusions: These results document the important role played by vitamin A in laryngeal development.
AB - Objectives: Our previous research demonstrated that vitamin A might be related to vocal fold development. The purpose of this study was to determine whether vitamin A deficiency affects prenatal laryngeal development in rats. Methods: Two considerations were necessary in designing a study using a rat model: for embryonic survival, vitamin A is necessary through day 10 of gestation, and laryngeal formation occurs primarily after day 11. Thus, we created a rat model that developed vitamin A deficiency after embryonic day 11. Ten pregnant rats (5 vitamin A-deficient rats and 5 control rats) were studied. Embryos were collected at embryonic day 18.5 and analyzed histologically. Results: Eighteen percent of the vitamin A-deficient embryos were alive and demonstrated laryngotracheal cartilage malformation, incomplete separation of the glottis, and/or laryngoesophageal clefts. Conclusions: These results document the important role played by vitamin A in laryngeal development.
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U2 - 10.1177/000348940711601011
DO - 10.1177/000348940711601011
M3 - Article
C2 - 17987785
AN - SCOPUS:35348899015
SN - 0003-4894
VL - 116
SP - 785
EP - 792
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
IS - 10
ER -