Abstract
Background. The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult. Methods. We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh. Results. After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases. Conclusion. Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.
| Original language | English |
|---|---|
| Pages (from-to) | 983-990 |
| Number of pages | 8 |
| Journal | Head and Neck |
| Volume | 30 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 01-08-2008 |
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All Science Journal Classification (ASJC) codes
- Otorhinolaryngology
Cite this
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Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis. / Takano, Toru; Higashiyama, Takuya; Uruno, Takashi; Yamada, Hiroya; Yoshida, Hiroshi; Miyauchi, Akira.
In: Head and Neck, Vol. 30, No. 8, 01.08.2008, p. 983-990.Research output: Contribution to journal › Article
TY - JOUR
T1 - Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis
AU - Takano, Toru
AU - Higashiyama, Takuya
AU - Uruno, Takashi
AU - Yamada, Hiroya
AU - Yoshida, Hiroshi
AU - Miyauchi, Akira
PY - 2008/8/1
Y1 - 2008/8/1
N2 - Background. The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult. Methods. We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh. Results. After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases. Conclusion. Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.
AB - Background. The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult. Methods. We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh. Results. After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases. Conclusion. Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.
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U2 - 10.1002/hed.20810
DO - 10.1002/hed.20810
M3 - Article
C2 - 18302262
AN - SCOPUS:50249157427
VL - 30
SP - 983
EP - 990
JO - Head and Neck
JF - Head and Neck
SN - 1043-3074
IS - 8
ER -