Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis

Toru Takano, Takuya Higashiyama, Takashi Uruno, Hiroya Yamada, Hiroshi Yoshida, Akira Miyauchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background. The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult. Methods. We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh. Results. After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases. Conclusion. Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.

Original languageEnglish
Pages (from-to)983-990
Number of pages8
JournalHead and Neck
Volume30
Issue number8
DOIs
Publication statusPublished - 01-08-2008
Externally publishedYes

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Needle Biopsy
Nucleic Acids
Galectin 3
Thyroid Gland
Blood Cells
Neoplasms
Messenger RNA
Antibodies
Nylons
Adenoma
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Takano, Toru ; Higashiyama, Takuya ; Uruno, Takashi ; Yamada, Hiroya ; Yoshida, Hiroshi ; Miyauchi, Akira. / Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis. In: Head and Neck. 2008 ; Vol. 30, No. 8. pp. 983-990.
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Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis. / Takano, Toru; Higashiyama, Takuya; Uruno, Takashi; Yamada, Hiroya; Yoshida, Hiroshi; Miyauchi, Akira.

In: Head and Neck, Vol. 30, No. 8, 01.08.2008, p. 983-990.

Research output: Contribution to journalArticle

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T1 - Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis

AU - Takano, Toru

AU - Higashiyama, Takuya

AU - Uruno, Takashi

AU - Yamada, Hiroya

AU - Yoshida, Hiroshi

AU - Miyauchi, Akira

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