Presence of prostacyclin receptor in arteriosclerotic human femoral artery

A. Muto, Y. Kondo, Y. Yamamoto, H. Yamada, O. Washimi, Y. Miyauchi, F. Kudo, A. Dardik, T. Nishibe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aim. Prostacyclin, which is mainly synthesized by vascular endothelial cells, exerts antiplatelet and smooth-muscle-relaxant effects, thereby maintaining cardiovascular homeostasis. Prostacyclin analogues have been clinically proven to improve ischemic symptoms and prevent the occurrence of vascular events in the lower extremities of patients with arteriosclerosis obliterans. We examined the presence of prostacyclin receptor (IP receptor) in an arteriosclerotic human femoral artery. Methods. Specimens of the femoral artery were obtained at the time of limb amputation from an 83-year-old woman. Atherosclerotic lesions and associated changes such as calcification were evident. The specimens were stained with hematoxylin and eosin, and processed for immunohistochemistry. Results. A monolayer of cells was observed on the luminal side of the femoral artery. Single immunohistochemistry showed the presence of the IP receptors on cells of the luminal side of the femoral artery. Triple-immunofluorescence staining revealed colocalization of IP-receptor-positive cells and cells positive for von Willebrand factor, a marker of vascular endothelial cells. Conclusion. We investigated the presence of the IP receptor in the human femoral artery immunohistochemically, and demonstrated their strong expression in endothelial cells. This finding suggests that prostacyclin or prostacyclin analogues may act on their receptors on endothelial cells in patients with arteriosclerosis obliterans.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalInternational Angiology
Volume25
Issue number2
Publication statusPublished - 01-06-2006

Fingerprint

Epoprostenol Receptors
Femoral Artery
Epoprostenol
Endothelial Cells
Arteriosclerosis Obliterans
Immunohistochemistry
von Willebrand Factor
Hematoxylin
Eosine Yellowish-(YS)
Amputation
Fluorescent Antibody Technique
Smooth Muscle
Blood Vessels
Lower Extremity
Homeostasis
Extremities
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Muto, A., Kondo, Y., Yamamoto, Y., Yamada, H., Washimi, O., Miyauchi, Y., ... Nishibe, T. (2006). Presence of prostacyclin receptor in arteriosclerotic human femoral artery. International Angiology, 25(2), 175-178.
Muto, A. ; Kondo, Y. ; Yamamoto, Y. ; Yamada, H. ; Washimi, O. ; Miyauchi, Y. ; Kudo, F. ; Dardik, A. ; Nishibe, T. / Presence of prostacyclin receptor in arteriosclerotic human femoral artery. In: International Angiology. 2006 ; Vol. 25, No. 2. pp. 175-178.
@article{6b2ff5984e9b4a9f8011c056a8cbd77f,
title = "Presence of prostacyclin receptor in arteriosclerotic human femoral artery",
abstract = "Aim. Prostacyclin, which is mainly synthesized by vascular endothelial cells, exerts antiplatelet and smooth-muscle-relaxant effects, thereby maintaining cardiovascular homeostasis. Prostacyclin analogues have been clinically proven to improve ischemic symptoms and prevent the occurrence of vascular events in the lower extremities of patients with arteriosclerosis obliterans. We examined the presence of prostacyclin receptor (IP receptor) in an arteriosclerotic human femoral artery. Methods. Specimens of the femoral artery were obtained at the time of limb amputation from an 83-year-old woman. Atherosclerotic lesions and associated changes such as calcification were evident. The specimens were stained with hematoxylin and eosin, and processed for immunohistochemistry. Results. A monolayer of cells was observed on the luminal side of the femoral artery. Single immunohistochemistry showed the presence of the IP receptors on cells of the luminal side of the femoral artery. Triple-immunofluorescence staining revealed colocalization of IP-receptor-positive cells and cells positive for von Willebrand factor, a marker of vascular endothelial cells. Conclusion. We investigated the presence of the IP receptor in the human femoral artery immunohistochemically, and demonstrated their strong expression in endothelial cells. This finding suggests that prostacyclin or prostacyclin analogues may act on their receptors on endothelial cells in patients with arteriosclerosis obliterans.",
author = "A. Muto and Y. Kondo and Y. Yamamoto and H. Yamada and O. Washimi and Y. Miyauchi and F. Kudo and A. Dardik and T. Nishibe",
year = "2006",
month = "6",
day = "1",
language = "English",
volume = "25",
pages = "175--178",
journal = "International Angiology",
issn = "0392-9590",
publisher = "Edizioni Minerva Medica S.p.A.",
number = "2",

}

Muto, A, Kondo, Y, Yamamoto, Y, Yamada, H, Washimi, O, Miyauchi, Y, Kudo, F, Dardik, A & Nishibe, T 2006, 'Presence of prostacyclin receptor in arteriosclerotic human femoral artery', International Angiology, vol. 25, no. 2, pp. 175-178.

Presence of prostacyclin receptor in arteriosclerotic human femoral artery. / Muto, A.; Kondo, Y.; Yamamoto, Y.; Yamada, H.; Washimi, O.; Miyauchi, Y.; Kudo, F.; Dardik, A.; Nishibe, T.

In: International Angiology, Vol. 25, No. 2, 01.06.2006, p. 175-178.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Presence of prostacyclin receptor in arteriosclerotic human femoral artery

AU - Muto, A.

AU - Kondo, Y.

AU - Yamamoto, Y.

AU - Yamada, H.

AU - Washimi, O.

AU - Miyauchi, Y.

AU - Kudo, F.

AU - Dardik, A.

AU - Nishibe, T.

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Aim. Prostacyclin, which is mainly synthesized by vascular endothelial cells, exerts antiplatelet and smooth-muscle-relaxant effects, thereby maintaining cardiovascular homeostasis. Prostacyclin analogues have been clinically proven to improve ischemic symptoms and prevent the occurrence of vascular events in the lower extremities of patients with arteriosclerosis obliterans. We examined the presence of prostacyclin receptor (IP receptor) in an arteriosclerotic human femoral artery. Methods. Specimens of the femoral artery were obtained at the time of limb amputation from an 83-year-old woman. Atherosclerotic lesions and associated changes such as calcification were evident. The specimens were stained with hematoxylin and eosin, and processed for immunohistochemistry. Results. A monolayer of cells was observed on the luminal side of the femoral artery. Single immunohistochemistry showed the presence of the IP receptors on cells of the luminal side of the femoral artery. Triple-immunofluorescence staining revealed colocalization of IP-receptor-positive cells and cells positive for von Willebrand factor, a marker of vascular endothelial cells. Conclusion. We investigated the presence of the IP receptor in the human femoral artery immunohistochemically, and demonstrated their strong expression in endothelial cells. This finding suggests that prostacyclin or prostacyclin analogues may act on their receptors on endothelial cells in patients with arteriosclerosis obliterans.

AB - Aim. Prostacyclin, which is mainly synthesized by vascular endothelial cells, exerts antiplatelet and smooth-muscle-relaxant effects, thereby maintaining cardiovascular homeostasis. Prostacyclin analogues have been clinically proven to improve ischemic symptoms and prevent the occurrence of vascular events in the lower extremities of patients with arteriosclerosis obliterans. We examined the presence of prostacyclin receptor (IP receptor) in an arteriosclerotic human femoral artery. Methods. Specimens of the femoral artery were obtained at the time of limb amputation from an 83-year-old woman. Atherosclerotic lesions and associated changes such as calcification were evident. The specimens were stained with hematoxylin and eosin, and processed for immunohistochemistry. Results. A monolayer of cells was observed on the luminal side of the femoral artery. Single immunohistochemistry showed the presence of the IP receptors on cells of the luminal side of the femoral artery. Triple-immunofluorescence staining revealed colocalization of IP-receptor-positive cells and cells positive for von Willebrand factor, a marker of vascular endothelial cells. Conclusion. We investigated the presence of the IP receptor in the human femoral artery immunohistochemically, and demonstrated their strong expression in endothelial cells. This finding suggests that prostacyclin or prostacyclin analogues may act on their receptors on endothelial cells in patients with arteriosclerosis obliterans.

UR - http://www.scopus.com/inward/record.url?scp=33745887465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745887465&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 175

EP - 178

JO - International Angiology

JF - International Angiology

SN - 0392-9590

IS - 2

ER -

Muto A, Kondo Y, Yamamoto Y, Yamada H, Washimi O, Miyauchi Y et al. Presence of prostacyclin receptor in arteriosclerotic human femoral artery. International Angiology. 2006 Jun 1;25(2):175-178.