TY - JOUR
T1 - Presence of spontaneous epithelial-mesenchymal plasticity in esophageal cancer
AU - Tsuchihashi, Kenji
AU - Hirata, Yuki
AU - Yamasaki, Juntaro
AU - Suina, Kentaro
AU - Tanoue, Kenro
AU - Yae, Toshifumi
AU - Masuda, Kenta
AU - Baba, Eishi
AU - Akashi, Koichi
AU - Kitagawa, Yuko
AU - Saya, Hideyuki
AU - Nagano, Osamu
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/7
Y1 - 2022/7
N2 - Epithelial-mesenchymal plasticity (EMP) refers to the reversible cellular transition between epithelial and mesenchymal status. Spontaneous EMP is also reported in breast and prostate cancer, leading to the acquisition of stem-cell properties and chemoresistance. However, the presence of spontaneous EMP is still not reported in esophageal cancer. We screened 11 esophageal squamous cancer cell (ESCC) cell lines by CD44 isoform expression. KYSE520 was found to comprise heterogenous populations consisting of CD44v+ and CD44v– subpopulations. CD44v+ and CD44v– cells showed the expression of epithelial and mesenchymal markers, respectively. Single-cell sorting of CD44v+ and CD44v– cells revealed both cells gave rise to cell populations consisting of CD44v+ and CD44v– cells, indicating CD44v+ epithelial-like and CD44v− mesenchymal-like cells can generate counterparts, respectively. The ablation of Epithelial splicing regulatory protein 1 (ESRP1), a major regulator of CD44 mRNA splicing, resulted in the shift from CD44v+ to CD44v– cells in KYSE520. However, the expression of epithelial-mesenchymal transition (EMT)-related markers or transcriptional factors were almost not affected, suggesting ESRP1 functions downstream of EMP. Our results revealed the presence of spontaneous EMP in esophageal cancer and KYSE520 is useful model to understand spontaneous EMP.
AB - Epithelial-mesenchymal plasticity (EMP) refers to the reversible cellular transition between epithelial and mesenchymal status. Spontaneous EMP is also reported in breast and prostate cancer, leading to the acquisition of stem-cell properties and chemoresistance. However, the presence of spontaneous EMP is still not reported in esophageal cancer. We screened 11 esophageal squamous cancer cell (ESCC) cell lines by CD44 isoform expression. KYSE520 was found to comprise heterogenous populations consisting of CD44v+ and CD44v– subpopulations. CD44v+ and CD44v– cells showed the expression of epithelial and mesenchymal markers, respectively. Single-cell sorting of CD44v+ and CD44v– cells revealed both cells gave rise to cell populations consisting of CD44v+ and CD44v– cells, indicating CD44v+ epithelial-like and CD44v− mesenchymal-like cells can generate counterparts, respectively. The ablation of Epithelial splicing regulatory protein 1 (ESRP1), a major regulator of CD44 mRNA splicing, resulted in the shift from CD44v+ to CD44v– cells in KYSE520. However, the expression of epithelial-mesenchymal transition (EMT)-related markers or transcriptional factors were almost not affected, suggesting ESRP1 functions downstream of EMP. Our results revealed the presence of spontaneous EMP in esophageal cancer and KYSE520 is useful model to understand spontaneous EMP.
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U2 - 10.1016/j.bbrep.2022.101246
DO - 10.1016/j.bbrep.2022.101246
M3 - Article
AN - SCOPUS:85126520852
SN - 2405-5808
VL - 30
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 101246
ER -
