Present and future of reverse genetics of rotavirus

Research output: Contribution to journalReview article

Abstract

Rotavirus is the leading pathogen for acute gastroenteritis in mammals and birds. Although the reverse genetics system has been utilized in many viruses, the system using a helper virus was developed for rotavirus in 2006. As a step for antigenic analysis of VP4 antigen of rotavirus, we prepared an infectious rotavirus with a spike protein VP4 having an antigenic mosaic by substituting one of the cross-reactive neutralization epitopes of a simian strain SA-11 with the corresponding one of a human strain DS-1. The future improvement and application of the rotavirus reverse genetics were discussed in this review.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalUirusu. Journal of virology
Volume59
Issue number1
DOIs
Publication statusPublished - 01-01-2009

Fingerprint

Reverse Genetics
Rotavirus
Helper Viruses
Gastroenteritis
Birds
Epitopes
Mammals
Viruses
Antigens
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{01bc957d289e402b98a5d7084a16ff98,
title = "Present and future of reverse genetics of rotavirus",
abstract = "Rotavirus is the leading pathogen for acute gastroenteritis in mammals and birds. Although the reverse genetics system has been utilized in many viruses, the system using a helper virus was developed for rotavirus in 2006. As a step for antigenic analysis of VP4 antigen of rotavirus, we prepared an infectious rotavirus with a spike protein VP4 having an antigenic mosaic by substituting one of the cross-reactive neutralization epitopes of a simian strain SA-11 with the corresponding one of a human strain DS-1. The future improvement and application of the rotavirus reverse genetics were discussed in this review.",
author = "Koki Taniguchi and Satoshi Komoto and Jun Sasaki and Masanori Kugita",
year = "2009",
month = "1",
day = "1",
doi = "10.2222/jsv.59.91",
language = "English",
volume = "59",
pages = "91--98",
journal = "Uirusu. Journal of virology",
issn = "0042-6857",
publisher = "Nihon Uirusu Gakkal/Japanese Society for Virology",
number = "1",

}

Present and future of reverse genetics of rotavirus. / Taniguchi, Koki; Komoto, Satoshi; Sasaki, Jun; Kugita, Masanori.

In: Uirusu. Journal of virology, Vol. 59, No. 1, 01.01.2009, p. 91-98.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Present and future of reverse genetics of rotavirus

AU - Taniguchi, Koki

AU - Komoto, Satoshi

AU - Sasaki, Jun

AU - Kugita, Masanori

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Rotavirus is the leading pathogen for acute gastroenteritis in mammals and birds. Although the reverse genetics system has been utilized in many viruses, the system using a helper virus was developed for rotavirus in 2006. As a step for antigenic analysis of VP4 antigen of rotavirus, we prepared an infectious rotavirus with a spike protein VP4 having an antigenic mosaic by substituting one of the cross-reactive neutralization epitopes of a simian strain SA-11 with the corresponding one of a human strain DS-1. The future improvement and application of the rotavirus reverse genetics were discussed in this review.

AB - Rotavirus is the leading pathogen for acute gastroenteritis in mammals and birds. Although the reverse genetics system has been utilized in many viruses, the system using a helper virus was developed for rotavirus in 2006. As a step for antigenic analysis of VP4 antigen of rotavirus, we prepared an infectious rotavirus with a spike protein VP4 having an antigenic mosaic by substituting one of the cross-reactive neutralization epitopes of a simian strain SA-11 with the corresponding one of a human strain DS-1. The future improvement and application of the rotavirus reverse genetics were discussed in this review.

UR - http://www.scopus.com/inward/record.url?scp=74249113467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74249113467&partnerID=8YFLogxK

U2 - 10.2222/jsv.59.91

DO - 10.2222/jsv.59.91

M3 - Review article

VL - 59

SP - 91

EP - 98

JO - Uirusu. Journal of virology

JF - Uirusu. Journal of virology

SN - 0042-6857

IS - 1

ER -