Pretreatment effects of jelly components on the sperm acrosome reaction and histone degradation in the starfish, Asterina pectinifera

Tosikazu Amano, Yoshihito Okita, Taei Matsui, Motonori Hoshi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Acrosome reaction (AR) and histone degradation (HD) of Asterina pectinifera sperm are induced by co-operation of ARIS and a diffusible fraction (M8) of egg jelly. Once sperm are treated with ARIS or M8 separately for several minutes, they do not undergo the AR in response to the egg jelly. Preincubation of sperm with M8 at 0°C is not effective to block the jelly-induced AR whereas inhibitory effects of ARIS remain at 0°C. Jelly-induced HD is inhibited by pretreatment of sperm with ARIS but is not affected by the incubation with M8. The blockage of the jelly-induced reactions, both AR and HD, by ARIS- or M8-pretreatment can be bypassed by ionophores, A23187 and monensin.

Original languageEnglish
Pages (from-to)268-273
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume187
Issue number1
DOIs
Publication statusPublished - 31-08-1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Pretreatment effects of jelly components on the sperm acrosome reaction and histone degradation in the starfish, Asterina pectinifera'. Together they form a unique fingerprint.

Cite this