Prevalence of CTX-M-type extended-spectrum β-lactamase-producing escherichia coli B2-O25-ST131 H30R among residents in nonacute care facilities in Japan

Kumiko Kawamura, Kengo Hayashi, Nao Matsuo, Kazuki Kitaoka, Kouji Kimura, Jun Ichi Wachino, Takaaki Kondo, Yoshitsugu Iinuma, Nobuo Murakami, Shuhei Fujimoto, Yoshichika Arakawa

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15 Citations (Scopus)

Abstract

We investigated the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli among 258 residents of long-term care facilities (LTCFs) in Japan. Out of 258 fecal samples collected from nine LTCFs between November 2015 and March 2017, we recovered 59 ESBL-producing E. Coli isolates. All isolates carried bla CTX-M genes, mainly bla CTX-M-27 (42.4%), bla CTX-M-14 (23.7%), and bla CTX-M-15 (18.6%). The isolates showed 7 serotypes (STs), including ST131 (n = 49, 83.1%) and ST38 (n = 4, 6.8%), and 47 (79.7%) out of 49 isolates belonging to ST131 were identified as H30R. The 59 ESBL producers were divided into four groups, B2 (86.4%), D (8.5%), A (3.4%), and C (1.7%); 44 (74.6%) were epidemic clone B2-O25-ST131 H30R, of which 21, 11, and 6 harbored bla CTX-M-27 , bla CTX-M-15 , and bla CTX-M-14 , respectively. Most plasmids were of IncF replicon types (n = 33), and 22 bla CTX-M-27 -carrying plasmids showed multiple replicon types, including IncFII, FIA, and FIB. The ESBL producers were susceptible to imipenem, amikacin, and fosfomycin, but resistant to ceftazidime (49.2%), and ciprofloxacin (88.1%); in particular, the isolates harboring the bla CTX-M-15 gene showed significantly high resistance rate to ceftazidime (p < 0.01). Our findings indicate that a considerable proportion of the examined LTCF residents carried ESBL-producing E. Coli isolates in feces and had high prevalence of epidemic clone B2-O25-ST131. Furthermore, continuous investigations would be very necessary to monitor actual carriage states of ESBL-producers among the LTCF residents from the viewpoint of both public health and healthcare viewpoints.

Original languageEnglish
Pages (from-to)1513-1520
Number of pages8
JournalMicrobial Drug Resistance
Volume24
Issue number10
DOIs
Publication statusPublished - 12-2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

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