Prevention of ischemic neuronal death by intravenous infusion of a ginseng saponin, ginsenoside Rb1, that upregulates Bcl-xL expression

Bo Zhang, Ryuji Hata, Pengxiang Zhu, Kohji Sato, Tong Chun Wen, Lihua Yang, Hiroko Fujita, Noriaki Mitsuda, Junya Tanaka, Keiichi Samukawa, Nobuji Maeda, Masahiro Sakanaka

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Almost all agents that exhibit neuroprotection when administered into the cerebral ventricles are ineffective or much less effective in rescuing damaged neurons when infused into the blood stream. Search for an intravenously infusible drug with a potent neuroprotective action is essential for the treatment of millions of patients suffering from acute brain diseases. Here, we report that postischemic intravenous infusion of a ginseng saponin, ginsenoside Rb1 (gRb1) (C54H92O23, molecular weight 1109.46) to stroke-prone spontaneously hypertensive rats with permanent occlusion of the middle cerebral artery distal to the striate branches significantly ameliorated ischemia-induced place navigation disability and caused an approximately 50% decrease in the volume of the cortical infarct lesion in comparison with vehicle-infused ischemic controls. In subsequent studies that focused on gRb1-induced expression of gene products responsible for neuronal death or survival, we showed that gRb1 stimulated the expression of the mitochondrion-associated antiapoptotic factor Bcl-xL in vitro and in vivo. Moreover, we revealed that a Stat5 responsive element in the bcl-x promoter became active in response to gRb1 treatment. Ginsenoside Rb1 appears to be a promising agent not only for the treatment of cerebral stroke, but also for the treatment of other diseases involving activation of mitochondrial cell death signaling.

Original languageEnglish
Pages (from-to)708-721
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Volume26
Issue number5
DOIs
Publication statusPublished - 01-05-2006

Fingerprint

Panax
Saponins
Intravenous Infusions
Up-Regulation
Stroke
Cerebral Ventricles
Middle Cerebral Artery Infarction
Brain Diseases
Acute Disease
Inbred SHR Rats
Therapeutics
Mitochondria
Cell Death
Ischemia
Molecular Weight
ginsenoside Rb1
Gene Expression
Neurons
Survival
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Zhang, Bo ; Hata, Ryuji ; Zhu, Pengxiang ; Sato, Kohji ; Wen, Tong Chun ; Yang, Lihua ; Fujita, Hiroko ; Mitsuda, Noriaki ; Tanaka, Junya ; Samukawa, Keiichi ; Maeda, Nobuji ; Sakanaka, Masahiro. / Prevention of ischemic neuronal death by intravenous infusion of a ginseng saponin, ginsenoside Rb1, that upregulates Bcl-xL expression. In: Journal of Cerebral Blood Flow and Metabolism. 2006 ; Vol. 26, No. 5. pp. 708-721.
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Zhang, B, Hata, R, Zhu, P, Sato, K, Wen, TC, Yang, L, Fujita, H, Mitsuda, N, Tanaka, J, Samukawa, K, Maeda, N & Sakanaka, M 2006, 'Prevention of ischemic neuronal death by intravenous infusion of a ginseng saponin, ginsenoside Rb1, that upregulates Bcl-xL expression', Journal of Cerebral Blood Flow and Metabolism, vol. 26, no. 5, pp. 708-721. https://doi.org/10.1038/sj.jcbfm.9600225

Prevention of ischemic neuronal death by intravenous infusion of a ginseng saponin, ginsenoside Rb1, that upregulates Bcl-xL expression. / Zhang, Bo; Hata, Ryuji; Zhu, Pengxiang; Sato, Kohji; Wen, Tong Chun; Yang, Lihua; Fujita, Hiroko; Mitsuda, Noriaki; Tanaka, Junya; Samukawa, Keiichi; Maeda, Nobuji; Sakanaka, Masahiro.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 26, No. 5, 01.05.2006, p. 708-721.

Research output: Contribution to journalArticle

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AU - Sato, Kohji

AU - Wen, Tong Chun

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AU - Fujita, Hiroko

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AU - Maeda, Nobuji

AU - Sakanaka, Masahiro

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