TY - JOUR
T1 - Process for immune defect and chromosomal translocation during early thymocyte development lacking ATM
AU - Isoda, Takeshi
AU - Takagi, Masatoshi
AU - Piao, Jinhua
AU - Nakagama, Shun
AU - Sato, Masaki
AU - Masuda, Kyoko
AU - Ikawa, Tomokatsu
AU - Azuma, Miyuki
AU - Morio, Tomohiro
AU - Kawamoto, Hiroshi
AU - Mizutani, Shuki
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (81300953, 81371947, 81171747, 31671087), Natural Science Foundation of JiangSu Province (BK20150555) and China Postdoctoral Science Foundation (2014T70982, 2012M521868, 2015M572814).
PY - 2012/7/26
Y1 - 2012/7/26
N2 - Immune defect in ataxia telangiectasia patients has been attributed to either the failure of V(D)J recombination or class-switch recombination, and the chromosomal translocation in their lymphoma often involves the TCR gene. The ATM-deficient mouse exhibits fewer CD4 and CD8 single-positive T cells because of a failure to develop from the CD4+CD8+double-positive phase to the single-positive phase. Although the occurrence of chromosome 14 translocations involving TCR-δ gene in ATM-deficient lymphomas suggests that these are early events in T-cell development, a thorough analysis focusing on early T-cell development has never been performed. Here we demonstrate that ATM-deficient mouse thymocytes are perturbed in passing through the β- or γδ-selection checkpoint, leading in part to the developmental failure of T cells. Detailed karyotype analysis using the in vitro thymocyte development system revealed that RAG-mediated TCR-α/δlocus breaks occur and are left unrepaired during the troublesome β- or γδ- selection checkpoints. By getting through these selection checkpoints, some of the clones with random or nonrandom chromosomal translocations involving TCR- α/δ locus are selectedandaccumulate. Thus, our study visualized the first step of multi-step evolutions toward lymphomagenesis inATM-deficient thymocytes associated with T-lymphopenia and immunodeficiency.
AB - Immune defect in ataxia telangiectasia patients has been attributed to either the failure of V(D)J recombination or class-switch recombination, and the chromosomal translocation in their lymphoma often involves the TCR gene. The ATM-deficient mouse exhibits fewer CD4 and CD8 single-positive T cells because of a failure to develop from the CD4+CD8+double-positive phase to the single-positive phase. Although the occurrence of chromosome 14 translocations involving TCR-δ gene in ATM-deficient lymphomas suggests that these are early events in T-cell development, a thorough analysis focusing on early T-cell development has never been performed. Here we demonstrate that ATM-deficient mouse thymocytes are perturbed in passing through the β- or γδ-selection checkpoint, leading in part to the developmental failure of T cells. Detailed karyotype analysis using the in vitro thymocyte development system revealed that RAG-mediated TCR-α/δlocus breaks occur and are left unrepaired during the troublesome β- or γδ- selection checkpoints. By getting through these selection checkpoints, some of the clones with random or nonrandom chromosomal translocations involving TCR- α/δ locus are selectedandaccumulate. Thus, our study visualized the first step of multi-step evolutions toward lymphomagenesis inATM-deficient thymocytes associated with T-lymphopenia and immunodeficiency.
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U2 - 10.1182/blood-2012-02-413195
DO - 10.1182/blood-2012-02-413195
M3 - Article
C2 - 22709691
AN - SCOPUS:84864441435
SN - 0006-4971
VL - 120
SP - 789
EP - 799
JO - Blood
JF - Blood
IS - 4
ER -