Process for immune defect and chromosomal translocation during early thymocyte development lacking ATM

Takeshi Isoda, Masatoshi Takagi, Jinhua Piao, Shun Nakagama, Masaki Sato, Kyoko Masuda, Tomokatsu Ikawa, Miyuki Azuma, Tomohiro Morio, Hiroshi Kawamoto, Shuki Mizutani

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Immune defect in ataxia telangiectasia patients has been attributed to either the failure of V(D)J recombination or class-switch recombination, and the chromosomal translocation in their lymphoma often involves the TCR gene. The ATM-deficient mouse exhibits fewer CD4 and CD8 single-positive T cells because of a failure to develop from the CD4 +CD8 +double-positive phase to the single-positive phase. Although the occurrence of chromosome 14 translocations involving TCR-δ gene in ATM-deficient lymphomas suggests that these are early events in T-cell development, a thorough analysis focusing on early T-cell development has never been performed. Here we demonstrate that ATM-deficient mouse thymocytes are perturbed in passing through the β- or γδ-selection checkpoint, leading in part to the developmental failure of T cells. Detailed karyotype analysis using the in vitro thymocyte development system revealed that RAG-mediated TCR-α/δlocus breaks occur and are left unrepaired during the troublesome β- or γδ- selection checkpoints. By getting through these selection checkpoints, some of the clones with random or nonrandom chromosomal translocations involving TCR- α/δ locus are selectedandaccumulate. Thus, our study visualized the first step of multi-step evolutions toward lymphomagenesis inATM-deficient thymocytes associated with T-lymphopenia and immunodeficiency.

Original languageEnglish
Pages (from-to)789-799
Number of pages11
JournalBlood
Volume120
Issue number4
DOIs
Publication statusPublished - 26-07-2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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