Process of tight junction recovery in the injured vocal fold epithelium: Morphological and paracellular permeability analysis

Ryo Suzuki, Tatsuya Katsuno, Yo Kishimoto, Ryosuke Nakamura, Masanobu Mizuta, Atsushi Suehiro, Masaru Yamashita, Tatsuo Nakamura, Ichiro Tateya, Koichi Omori

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives/Hypothesis: The vocal fold epithelium that includes tight junction (TJ)-based barrier function protects underlying connective tissues from external insults. TJs play an important role to control paracellular permeability of not only solutes but also ions, and preserve the vocal fold homeostasis. However, the distribution of TJs and paracellular diffusion barrier across the entire vocal fold epithelium are still unknown. The aim of this study was to identify the distribution of TJs in the vocal fold epithelium and to characterize the recovery process of TJ-based paracellular diffusion barrier in a rat model of vocal fold injury. Study Design: Animal experiments with controls. Methods: Normal and vocal fold–injured rats were used. Larynges were harvested for immunohistochemical examination of TJ proteins. For functional analysis, a tracer permeability assay was performed using EZ-Link Sulfo-NHS-LC-Biotin. Results: TJ proteins occludin and zonula occludens 1 signals were localized to the junctional regions of the most luminal cell layers of the vocal fold epithelium. The injured region had been recovered with epithelium at 5 days postinjury, but the paracellular diffusion barrier assays revealed that biotinylation reagents diffused into the lamina propria at 5 days postinjury, and were blocked at the epithelium at 14 and 28 days postinjury. Conclusions: It was strongly suggested that TJs in the vocal fold epithelium exist at the junctional regions of the first layer of stratified squamous epithelium. TJ-based paracellular diffusion barrier following vocal fold injury is recovered by 14 days postinjury, and this period corresponds with the time course of structural changes in the regenerating epithelium layer. Level of Evidence: NA. Laryngoscope, 128:E150–E156, 2018.

Original languageEnglish
Pages (from-to)E150-E156
JournalLaryngoscope
Volume128
Issue number4
DOIs
Publication statusPublished - 04-2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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