Processing of nerve growth factor: The role of basic amino acid clusters in the pro-region

Akihiro Mouri, Hiroshi Nomoto, Shoei Furukawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Neurotrophins are synthesized first as precursors called pro-neurotrophins, and their propeptides are then proteolytically removed to form mature neurotrophins. However, a significant proportion of total neurotrophins has been shown to be secreted as pro-neurotrophins. Furthermore, pro- and mature neurotrophins have been shown to elicit opposite effects on cell survival. Thus, the processing step of neurotrophins is very important. In order to understand the mechanism of neurotrophin processing, we focused on the two basic amino acid clusters in the pro-region of nerve growth factor (NGF). Various NGFs mutated at basic amino acids in the pro-region were introduced in COS7 and PC12 cells. The results indicated that these basic amino acid clusters were actually cleaved in the cells by furin, but that their cleavage contributed little to the production of mature NGF. However, one of the two sites was considered to contribute to mature NGF production depending on conditions used.

Original languageEnglish
Pages (from-to)1056-1062
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number4
DOIs
Publication statusPublished - 23-02-2007

Fingerprint

Basic Amino Acids
Nerve Growth Factors
Nerve Growth Factor
Processing
Furin
PC12 Cells
Cell Survival
Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{1bd20798602a4542a99ac4928b885642,
title = "Processing of nerve growth factor: The role of basic amino acid clusters in the pro-region",
abstract = "Neurotrophins are synthesized first as precursors called pro-neurotrophins, and their propeptides are then proteolytically removed to form mature neurotrophins. However, a significant proportion of total neurotrophins has been shown to be secreted as pro-neurotrophins. Furthermore, pro- and mature neurotrophins have been shown to elicit opposite effects on cell survival. Thus, the processing step of neurotrophins is very important. In order to understand the mechanism of neurotrophin processing, we focused on the two basic amino acid clusters in the pro-region of nerve growth factor (NGF). Various NGFs mutated at basic amino acids in the pro-region were introduced in COS7 and PC12 cells. The results indicated that these basic amino acid clusters were actually cleaved in the cells by furin, but that their cleavage contributed little to the production of mature NGF. However, one of the two sites was considered to contribute to mature NGF production depending on conditions used.",
author = "Akihiro Mouri and Hiroshi Nomoto and Shoei Furukawa",
year = "2007",
month = "2",
day = "23",
doi = "10.1016/j.bbrc.2006.12.136",
language = "English",
volume = "353",
pages = "1056--1062",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

Processing of nerve growth factor : The role of basic amino acid clusters in the pro-region. / Mouri, Akihiro; Nomoto, Hiroshi; Furukawa, Shoei.

In: Biochemical and Biophysical Research Communications, Vol. 353, No. 4, 23.02.2007, p. 1056-1062.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Processing of nerve growth factor

T2 - The role of basic amino acid clusters in the pro-region

AU - Mouri, Akihiro

AU - Nomoto, Hiroshi

AU - Furukawa, Shoei

PY - 2007/2/23

Y1 - 2007/2/23

N2 - Neurotrophins are synthesized first as precursors called pro-neurotrophins, and their propeptides are then proteolytically removed to form mature neurotrophins. However, a significant proportion of total neurotrophins has been shown to be secreted as pro-neurotrophins. Furthermore, pro- and mature neurotrophins have been shown to elicit opposite effects on cell survival. Thus, the processing step of neurotrophins is very important. In order to understand the mechanism of neurotrophin processing, we focused on the two basic amino acid clusters in the pro-region of nerve growth factor (NGF). Various NGFs mutated at basic amino acids in the pro-region were introduced in COS7 and PC12 cells. The results indicated that these basic amino acid clusters were actually cleaved in the cells by furin, but that their cleavage contributed little to the production of mature NGF. However, one of the two sites was considered to contribute to mature NGF production depending on conditions used.

AB - Neurotrophins are synthesized first as precursors called pro-neurotrophins, and their propeptides are then proteolytically removed to form mature neurotrophins. However, a significant proportion of total neurotrophins has been shown to be secreted as pro-neurotrophins. Furthermore, pro- and mature neurotrophins have been shown to elicit opposite effects on cell survival. Thus, the processing step of neurotrophins is very important. In order to understand the mechanism of neurotrophin processing, we focused on the two basic amino acid clusters in the pro-region of nerve growth factor (NGF). Various NGFs mutated at basic amino acids in the pro-region were introduced in COS7 and PC12 cells. The results indicated that these basic amino acid clusters were actually cleaved in the cells by furin, but that their cleavage contributed little to the production of mature NGF. However, one of the two sites was considered to contribute to mature NGF production depending on conditions used.

UR - http://www.scopus.com/inward/record.url?scp=33846126878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846126878&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.12.136

DO - 10.1016/j.bbrc.2006.12.136

M3 - Article

C2 - 17207774

AN - SCOPUS:33846126878

VL - 353

SP - 1056

EP - 1062

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -