Production of α,ω-alkanediols using Escherichia coli expressing a cytochrome P450 from Acinetobacter sp. OC4

Tadashi Fujii, Tatsuya Narikawa, Futoshi Sumisa, Akira Arisawa, Koji Takeda, Junichi Kato

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from n-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in E. coli for the first time. Further, our biotransformation system showed α,ω-diterminal oxidation activity of n-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of α,ω-alkanediols from n-alkanes or 1-alkanols.

Original languageEnglish
Pages (from-to)1379-1385
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number6
DOIs
Publication statusPublished - 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Medicine

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