Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

Takayuki Otani; Toshihiro Hashizume; Tadahiro Nagaoka; Tomoko Fukuda; Careen K. Tang; David S. Salomon; Masaharu Seno

doi:10.1007/s10529-009-0160-9

Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

Takayuki Otani, Toshihiro Hashizume, Tadahiro Nagaoka, Tomoko Fukuda, Careen K. Tang, David S. Salomon, Masaharu Seno

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to approximately 50% of the conventional Fc-fusion design without an Fc domain of IgG. The refolded dimers could be easily analyzed and characterized by SDS-PAGE. Hinge-tagged soluble ErbBs demonstrated significant affinity for betacellulin and heregulin. The IgG hinge-tag should be a simple method to design soluble dimers that would be useful for high throughput screening of ligands, antagonists or derivatives.

Original language	English
Pages (from-to)	361-366
Number of pages	6
Journal	Biotechnology Letters
Volume	32
Issue number	3
DOIs	https://doi.org/10.1007/s10529-009-0160-9
Publication status	Published - 01-02-2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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abstract = "The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to approximately 50% of the conventional Fc-fusion design without an Fc domain of IgG. The refolded dimers could be easily analyzed and characterized by SDS-PAGE. Hinge-tagged soluble ErbBs demonstrated significant affinity for betacellulin and heregulin. The IgG hinge-tag should be a simple method to design soluble dimers that would be useful for high throughput screening of ligands, antagonists or derivatives.",

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T1 - Production of biologically active IgG hinge-tag soluble epidermal growth factor receptors (ErbB)

AU - Otani, Takayuki

AU - Hashizume, Toshihiro

AU - Nagaoka, Tadahiro

AU - Fukuda, Tomoko

AU - Tang, Careen K.

AU - Salomon, David S.

AU - Seno, Masaharu

PY - 2010/2/1

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