TY - JOUR
T1 - Production of IL-10 and IL-12 in CD40 and interleukin 4-activated mononuclear cells from patients with Graves' disease
AU - Itoh, M.
AU - Uchimura, K.
AU - Makino, M.
AU - Kobayashi, T.
AU - Hayashi, R.
AU - Nagata, M.
AU - Kakizawa, H.
AU - Fujiwara, K.
AU - Nagasaka, A.
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan (Grant-in-Aid for Scientific Research (C) No. 09671081) and from Fujita Health University. The authors thank Ms N. Takekawa for her secretarial assistance.
PY - 2000/6
Y1 - 2000/6
N2 - We investigated the effect of T cell-dependent B cell activation on the production of IL-10 and IL-12 by peripheral blood mononuclear cells (PBMCs) obtained from patients with Graves' disease vs Hashimoto's thyroiditis, type 1 diabetes or normal controls. Incubation of PBMCs, from each of the subject groups, with a combination of anti-CD40 monoclonal antibodies and interleukin 4 (IL-4)-activated B cells, as shown by an increased level of soluble CD23. There was also a notable increase in the number of CD23+ cells in PBMCs from patients with Graves' disease as compared to the other subject groups. This combination of B cell stimulants increased production of IL-10 in PBMCs obtained from patients with Graves' disease relative to those patients with Hashimoto's thyroiditis, type 1 diabetes, or the control subjects. The production of IL-12 showed wide variation that depended on the basal IL-12 level. In subjects with a low basal IL-12 level there was a positive correlation between the production of IL-12 and that of IL-10 from PBMCs stimulated with anti-CD40 antibodies plus IL-4. On the contrary, in the patients with a high basal IL-12 level, no change or a decrease of IL-12 production was observed after the stimulation. Thus, T cell-dependent B cell activation via a CD40 pathway triggers the overproduction of IL-10 and overcome the effect of IL-12 to shift the Th1/Th2 balance to Th2 dominance in patients with Graves' disease but not in Hashimoto's thyroiditis or type 1 diabetes. (C) 2000 Academic Press.
AB - We investigated the effect of T cell-dependent B cell activation on the production of IL-10 and IL-12 by peripheral blood mononuclear cells (PBMCs) obtained from patients with Graves' disease vs Hashimoto's thyroiditis, type 1 diabetes or normal controls. Incubation of PBMCs, from each of the subject groups, with a combination of anti-CD40 monoclonal antibodies and interleukin 4 (IL-4)-activated B cells, as shown by an increased level of soluble CD23. There was also a notable increase in the number of CD23+ cells in PBMCs from patients with Graves' disease as compared to the other subject groups. This combination of B cell stimulants increased production of IL-10 in PBMCs obtained from patients with Graves' disease relative to those patients with Hashimoto's thyroiditis, type 1 diabetes, or the control subjects. The production of IL-12 showed wide variation that depended on the basal IL-12 level. In subjects with a low basal IL-12 level there was a positive correlation between the production of IL-12 and that of IL-10 from PBMCs stimulated with anti-CD40 antibodies plus IL-4. On the contrary, in the patients with a high basal IL-12 level, no change or a decrease of IL-12 production was observed after the stimulation. Thus, T cell-dependent B cell activation via a CD40 pathway triggers the overproduction of IL-10 and overcome the effect of IL-12 to shift the Th1/Th2 balance to Th2 dominance in patients with Graves' disease but not in Hashimoto's thyroiditis or type 1 diabetes. (C) 2000 Academic Press.
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U2 - 10.1006/cyto.1999.0659
DO - 10.1006/cyto.1999.0659
M3 - Article
C2 - 10843746
AN - SCOPUS:0033936682
SN - 1043-4666
VL - 12
SP - 688
EP - 693
JO - Cytokine
JF - Cytokine
IS - 6
ER -
