Production of murine collagen-induced arthritis using Klebsiella pneumoniae O3 lipopolysaccharide as a potent immunological adjuvant

Kazuko Takahashi, Yutaka Kato, Tsuyoshi Sugiyama, Naoki Koide, Makoto Kawai, Masako Fukada, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Collagen-induced arthritis (CIA) was produced in mice with non H-2(q) and H-2(r) haplotypes by repeated immunization of porcine type-II collagen (CH) together with Klebsiella O3 lipopolysaccharide (KO3 LPS) as an immunological adjuvant. Histological changes that appeared in joints of repeatedly immunized mice were characterized by destruction of normal joint structure, synovial hyperplasia with proliferation of synovial cells, and infiltration of inflammatory cells. No such lesions were produced in mice receiving repeated injections of CII alone or KO3 LPS alone. Development of the humoral antibody and the delayed-type hypersensitivity to CH was exclusively found in mice immunized with the mixture of CII and KO3 LPS. It was therefore suggested that arthritis lesions induced by repeated immunization with the mixture of CII and KO3 LPS might be caused by an autoimmune mechanism, and that the experimental model might be useful for characterization of human rheumatoid arthritis (RA).

Original languageEnglish
Pages (from-to)795-801
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume43
Issue number8
DOIs
Publication statusPublished - 01-01-1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Production of murine collagen-induced arthritis using Klebsiella pneumoniae O3 lipopolysaccharide as a potent immunological adjuvant'. Together they form a unique fingerprint.

  • Cite this