TY - JOUR
T1 - Production of nitric oxide from endothelial cells by 31-amino-acid- length endothelin-1, a novel vasoconstrictive product by human chymase
AU - Niwa, Yasuharu
AU - Nagata, Noriko
AU - Oka, Makiko
AU - Toyoshima, Toshihiro
AU - Akiyoshi, Hiroko
AU - Wada, Tomoko
AU - Nakaya, Yutaka
PY - 2000/7/21
Y1 - 2000/7/21
N2 - Human chymase selectively converts big endothelin (ET)-1 to 31-amino- acid-length ET-1 [ET-1(1-31)]. In this study we examined effect of ET-1(1-31) on endothelial function. ET-1 (1-31) evoked contraction in a concentration- dependent manner at > 10-8 M, which was about 10 times weaker than that of conventional ET-1 [ET-1(1-21)]. BQ485, an ETA receptor antagonist, completely abolished ET-1(1-31)-induced contraction, but BQ788, an ETB receptor antagonist, slightly enhanced it, suggesting that ET-1(1-31) relaxes artery via endothelium. On endothelial cells, ET-1(1-21) and ET-1(1-31) increased [Ca2+]i and produced NO, both of which were significantly inhibited by BQ788 and not by BQ485. These results indicate that ET-1(1-31) increased [Ca2+]i and produced NO in endothelial cells through ETB receptor similarly with ET-1(1-21), although slight difference in effect on smooth muscle cells. (C) 2000 Elsevier Science Inc.
AB - Human chymase selectively converts big endothelin (ET)-1 to 31-amino- acid-length ET-1 [ET-1(1-31)]. In this study we examined effect of ET-1(1-31) on endothelial function. ET-1 (1-31) evoked contraction in a concentration- dependent manner at > 10-8 M, which was about 10 times weaker than that of conventional ET-1 [ET-1(1-21)]. BQ485, an ETA receptor antagonist, completely abolished ET-1(1-31)-induced contraction, but BQ788, an ETB receptor antagonist, slightly enhanced it, suggesting that ET-1(1-31) relaxes artery via endothelium. On endothelial cells, ET-1(1-21) and ET-1(1-31) increased [Ca2+]i and produced NO, both of which were significantly inhibited by BQ788 and not by BQ485. These results indicate that ET-1(1-31) increased [Ca2+]i and produced NO in endothelial cells through ETB receptor similarly with ET-1(1-21), although slight difference in effect on smooth muscle cells. (C) 2000 Elsevier Science Inc.
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U2 - 10.1016/S0024-3205(00)00700-1
DO - 10.1016/S0024-3205(00)00700-1
M3 - Article
C2 - 10954044
AN - SCOPUS:0034698229
SN - 0024-3205
VL - 67
SP - 1103
EP - 1109
JO - Life Sciences
JF - Life Sciences
IS - 9
ER -