Production of reactive persulfide species in chronic obstructive pulmonary disease

Tadahisa Numakura, Hisatoshi Sugiura, Takaaki Akaike, Tomoaki Ida, Shigemoto Fujii, Akira Koarai, Mitsuhiro Yamada, Katsuhiro Onodera, Yuichiro Hashimoto, Rie Tanaka, Kei Sato, Yutaka Shishikura, Taizou Hirano, Satoru Yanagisawa, Naoya Fujino, Tatsuma Okazaki, Tsutomu Tamada, Yasushi Hoshikawa, Yoshinori Okada, Masakazu Ichinose

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background Oxidative stress is a major aetiological factor driving chronic obstructive pulmonary disease (COPD). Recently recognised as potent antioxidants, reactive persulfide and polysulfide species are biosynthesised by cystathionine β-synthase and cystathionine Î 3-lyase. The production of reactive persulfide and polysulfide species in the lungs of patients with COPD remain unknown. Objectives The aim of this study was to examine the production of reactive persulfides and polysulfides, such as glutathione persulfide (GSSH), cysteine persulfide (CysSSH) and glutathione trisulfide (GSSSH), in lung-resident cells and epithelial lining fluid (ELF) obtained from patients with mild to moderate COPD. Methods Lung tissues, primary lung cells, ELF and sputum were obtained. The amounts of reactive persulfides and polysulfides in the cells and ELF were measured by liquid chromatography-tandem mass spectrometry with β-(4-hydroxyphenyl) ethyl iodoacetamide as a trapping agent for hydroper/polysulfides. The amounts of synthases in the lung tissues, sputum and primary cells were quantified. Results The amounts of GSSH, CysSSH and GSSSH were decreased in the lung cells and ELF from patients with COPD. The amounts of reactive persulfides and polysulfides in the lung cells had a positive correlation with the degree of airflow limitation. By contrast, the amounts of the synthases were increased in the lung tissues and sputum cells of patients with COPD. Conclusions We have identified a decrease in reactive persulfide and polysulfide species in the lungs of patients with COPD. These data suggest that the newly detected antioxidants reactive persulfides and polysulfides could be associated with the redox balance in the lungs of patients with COPD.

Original languageEnglish
Pages (from-to)1074-1083
Number of pages10
JournalThorax
Volume72
Issue number12
DOIs
Publication statusPublished - 01-12-2017

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Chronic Obstructive Pulmonary Disease
Lung
Sputum
Epithelial Cells
Cystathionine
Antioxidants
persulfides
Lyases
polysulfide
Tandem Mass Spectrometry
Liquid Chromatography
Oxidation-Reduction
Glutathione
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Numakura, T., Sugiura, H., Akaike, T., Ida, T., Fujii, S., Koarai, A., ... Ichinose, M. (2017). Production of reactive persulfide species in chronic obstructive pulmonary disease. Thorax, 72(12), 1074-1083. https://doi.org/10.1136/thoraxjnl-2016-209359
Numakura, Tadahisa ; Sugiura, Hisatoshi ; Akaike, Takaaki ; Ida, Tomoaki ; Fujii, Shigemoto ; Koarai, Akira ; Yamada, Mitsuhiro ; Onodera, Katsuhiro ; Hashimoto, Yuichiro ; Tanaka, Rie ; Sato, Kei ; Shishikura, Yutaka ; Hirano, Taizou ; Yanagisawa, Satoru ; Fujino, Naoya ; Okazaki, Tatsuma ; Tamada, Tsutomu ; Hoshikawa, Yasushi ; Okada, Yoshinori ; Ichinose, Masakazu. / Production of reactive persulfide species in chronic obstructive pulmonary disease. In: Thorax. 2017 ; Vol. 72, No. 12. pp. 1074-1083.
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abstract = "Background Oxidative stress is a major aetiological factor driving chronic obstructive pulmonary disease (COPD). Recently recognised as potent antioxidants, reactive persulfide and polysulfide species are biosynthesised by cystathionine β-synthase and cystathionine {\^I} 3-lyase. The production of reactive persulfide and polysulfide species in the lungs of patients with COPD remain unknown. Objectives The aim of this study was to examine the production of reactive persulfides and polysulfides, such as glutathione persulfide (GSSH), cysteine persulfide (CysSSH) and glutathione trisulfide (GSSSH), in lung-resident cells and epithelial lining fluid (ELF) obtained from patients with mild to moderate COPD. Methods Lung tissues, primary lung cells, ELF and sputum were obtained. The amounts of reactive persulfides and polysulfides in the cells and ELF were measured by liquid chromatography-tandem mass spectrometry with β-(4-hydroxyphenyl) ethyl iodoacetamide as a trapping agent for hydroper/polysulfides. The amounts of synthases in the lung tissues, sputum and primary cells were quantified. Results The amounts of GSSH, CysSSH and GSSSH were decreased in the lung cells and ELF from patients with COPD. The amounts of reactive persulfides and polysulfides in the lung cells had a positive correlation with the degree of airflow limitation. By contrast, the amounts of the synthases were increased in the lung tissues and sputum cells of patients with COPD. Conclusions We have identified a decrease in reactive persulfide and polysulfide species in the lungs of patients with COPD. These data suggest that the newly detected antioxidants reactive persulfides and polysulfides could be associated with the redox balance in the lungs of patients with COPD.",
author = "Tadahisa Numakura and Hisatoshi Sugiura and Takaaki Akaike and Tomoaki Ida and Shigemoto Fujii and Akira Koarai and Mitsuhiro Yamada and Katsuhiro Onodera and Yuichiro Hashimoto and Rie Tanaka and Kei Sato and Yutaka Shishikura and Taizou Hirano and Satoru Yanagisawa and Naoya Fujino and Tatsuma Okazaki and Tsutomu Tamada and Yasushi Hoshikawa and Yoshinori Okada and Masakazu Ichinose",
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Numakura, T, Sugiura, H, Akaike, T, Ida, T, Fujii, S, Koarai, A, Yamada, M, Onodera, K, Hashimoto, Y, Tanaka, R, Sato, K, Shishikura, Y, Hirano, T, Yanagisawa, S, Fujino, N, Okazaki, T, Tamada, T, Hoshikawa, Y, Okada, Y & Ichinose, M 2017, 'Production of reactive persulfide species in chronic obstructive pulmonary disease', Thorax, vol. 72, no. 12, pp. 1074-1083. https://doi.org/10.1136/thoraxjnl-2016-209359

Production of reactive persulfide species in chronic obstructive pulmonary disease. / Numakura, Tadahisa; Sugiura, Hisatoshi; Akaike, Takaaki; Ida, Tomoaki; Fujii, Shigemoto; Koarai, Akira; Yamada, Mitsuhiro; Onodera, Katsuhiro; Hashimoto, Yuichiro; Tanaka, Rie; Sato, Kei; Shishikura, Yutaka; Hirano, Taizou; Yanagisawa, Satoru; Fujino, Naoya; Okazaki, Tatsuma; Tamada, Tsutomu; Hoshikawa, Yasushi; Okada, Yoshinori; Ichinose, Masakazu.

In: Thorax, Vol. 72, No. 12, 01.12.2017, p. 1074-1083.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Production of reactive persulfide species in chronic obstructive pulmonary disease

AU - Numakura, Tadahisa

AU - Sugiura, Hisatoshi

AU - Akaike, Takaaki

AU - Ida, Tomoaki

AU - Fujii, Shigemoto

AU - Koarai, Akira

AU - Yamada, Mitsuhiro

AU - Onodera, Katsuhiro

AU - Hashimoto, Yuichiro

AU - Tanaka, Rie

AU - Sato, Kei

AU - Shishikura, Yutaka

AU - Hirano, Taizou

AU - Yanagisawa, Satoru

AU - Fujino, Naoya

AU - Okazaki, Tatsuma

AU - Tamada, Tsutomu

AU - Hoshikawa, Yasushi

AU - Okada, Yoshinori

AU - Ichinose, Masakazu

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Background Oxidative stress is a major aetiological factor driving chronic obstructive pulmonary disease (COPD). Recently recognised as potent antioxidants, reactive persulfide and polysulfide species are biosynthesised by cystathionine β-synthase and cystathionine Î 3-lyase. The production of reactive persulfide and polysulfide species in the lungs of patients with COPD remain unknown. Objectives The aim of this study was to examine the production of reactive persulfides and polysulfides, such as glutathione persulfide (GSSH), cysteine persulfide (CysSSH) and glutathione trisulfide (GSSSH), in lung-resident cells and epithelial lining fluid (ELF) obtained from patients with mild to moderate COPD. Methods Lung tissues, primary lung cells, ELF and sputum were obtained. The amounts of reactive persulfides and polysulfides in the cells and ELF were measured by liquid chromatography-tandem mass spectrometry with β-(4-hydroxyphenyl) ethyl iodoacetamide as a trapping agent for hydroper/polysulfides. The amounts of synthases in the lung tissues, sputum and primary cells were quantified. Results The amounts of GSSH, CysSSH and GSSSH were decreased in the lung cells and ELF from patients with COPD. The amounts of reactive persulfides and polysulfides in the lung cells had a positive correlation with the degree of airflow limitation. By contrast, the amounts of the synthases were increased in the lung tissues and sputum cells of patients with COPD. Conclusions We have identified a decrease in reactive persulfide and polysulfide species in the lungs of patients with COPD. These data suggest that the newly detected antioxidants reactive persulfides and polysulfides could be associated with the redox balance in the lungs of patients with COPD.

AB - Background Oxidative stress is a major aetiological factor driving chronic obstructive pulmonary disease (COPD). Recently recognised as potent antioxidants, reactive persulfide and polysulfide species are biosynthesised by cystathionine β-synthase and cystathionine Î 3-lyase. The production of reactive persulfide and polysulfide species in the lungs of patients with COPD remain unknown. Objectives The aim of this study was to examine the production of reactive persulfides and polysulfides, such as glutathione persulfide (GSSH), cysteine persulfide (CysSSH) and glutathione trisulfide (GSSSH), in lung-resident cells and epithelial lining fluid (ELF) obtained from patients with mild to moderate COPD. Methods Lung tissues, primary lung cells, ELF and sputum were obtained. The amounts of reactive persulfides and polysulfides in the cells and ELF were measured by liquid chromatography-tandem mass spectrometry with β-(4-hydroxyphenyl) ethyl iodoacetamide as a trapping agent for hydroper/polysulfides. The amounts of synthases in the lung tissues, sputum and primary cells were quantified. Results The amounts of GSSH, CysSSH and GSSSH were decreased in the lung cells and ELF from patients with COPD. The amounts of reactive persulfides and polysulfides in the lung cells had a positive correlation with the degree of airflow limitation. By contrast, the amounts of the synthases were increased in the lung tissues and sputum cells of patients with COPD. Conclusions We have identified a decrease in reactive persulfide and polysulfide species in the lungs of patients with COPD. These data suggest that the newly detected antioxidants reactive persulfides and polysulfides could be associated with the redox balance in the lungs of patients with COPD.

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U2 - 10.1136/thoraxjnl-2016-209359

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Numakura T, Sugiura H, Akaike T, Ida T, Fujii S, Koarai A et al. Production of reactive persulfide species in chronic obstructive pulmonary disease. Thorax. 2017 Dec 1;72(12):1074-1083. https://doi.org/10.1136/thoraxjnl-2016-209359