Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry

Kazuki Nakajima, Ayako Kohyama-Koganeya, Yoshio Hirabayashi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Cell membrane surface glycosphingolipids (GSLs), which are synthesized from glucosylceramide (GlcCer) by the enzymatic action of glucosylceramide synthase (GlcT-1), play fundamental roles in many cellular processes. Recent studies have shown that in adipose tissue, GlcCer is involved in the regulation of energy (fat and sugar) metabolism and that GlcCer acts as a glucose donor in cholesteryl glucoside biosynthesis. To investigate the physiological functions of GlcT-1 and GlcCer, a method to profile GlcCer species by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been recently established. The method is based on a combination analysis of retention times in hydrophilic liquid chromatography (HILIC) with specified head group survey, such as neutral-loss and/or precursor-ion scans. Here, the application of this method for profiling GlcCer species in Drosophila melanogaster fat bodies in which Drosophila GLCT-1 (dGlcT-1) expression was manipulated is described, and the implications of these studies in the context of energy metabolism and obesity are discussed.

Original languageEnglish
Title of host publicationGlycoscience
Subtitle of host publicationBiology and Medicine
PublisherSpringer Japan
Pages565-571
Number of pages7
ISBN (Electronic)9784431548416
ISBN (Print)9784431548409
DOIs
Publication statusPublished - 01-01-2015

Fingerprint

Glucosylceramides
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
ceramide glucosyltransferase
Fats
Electrospray ionization
Glycosphingolipids
Fat Body
Electrospray Ionization Mass Spectrometry
Biosynthesis
Cell membranes
Drosophila melanogaster
Metabolism
Sugars
Energy Metabolism
Drosophila
Adipose Tissue
Obesity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nakajima, K., Kohyama-Koganeya, A., & Hirabayashi, Y. (2015). Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry. In Glycoscience: Biology and Medicine (pp. 565-571). Springer Japan. https://doi.org/10.1007/978-4-431-54841-6_123
Nakajima, Kazuki ; Kohyama-Koganeya, Ayako ; Hirabayashi, Yoshio. / Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry. Glycoscience: Biology and Medicine. Springer Japan, 2015. pp. 565-571
@inbook{6fdb0f0798be475f987f5aac768c1a15,
title = "Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry",
abstract = "Cell membrane surface glycosphingolipids (GSLs), which are synthesized from glucosylceramide (GlcCer) by the enzymatic action of glucosylceramide synthase (GlcT-1), play fundamental roles in many cellular processes. Recent studies have shown that in adipose tissue, GlcCer is involved in the regulation of energy (fat and sugar) metabolism and that GlcCer acts as a glucose donor in cholesteryl glucoside biosynthesis. To investigate the physiological functions of GlcT-1 and GlcCer, a method to profile GlcCer species by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been recently established. The method is based on a combination analysis of retention times in hydrophilic liquid chromatography (HILIC) with specified head group survey, such as neutral-loss and/or precursor-ion scans. Here, the application of this method for profiling GlcCer species in Drosophila melanogaster fat bodies in which Drosophila GLCT-1 (dGlcT-1) expression was manipulated is described, and the implications of these studies in the context of energy metabolism and obesity are discussed.",
author = "Kazuki Nakajima and Ayako Kohyama-Koganeya and Yoshio Hirabayashi",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/978-4-431-54841-6_123",
language = "English",
isbn = "9784431548409",
pages = "565--571",
booktitle = "Glycoscience",
publisher = "Springer Japan",
address = "Japan",

}

Nakajima, K, Kohyama-Koganeya, A & Hirabayashi, Y 2015, Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry. in Glycoscience: Biology and Medicine. Springer Japan, pp. 565-571. https://doi.org/10.1007/978-4-431-54841-6_123

Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry. / Nakajima, Kazuki; Kohyama-Koganeya, Ayako; Hirabayashi, Yoshio.

Glycoscience: Biology and Medicine. Springer Japan, 2015. p. 565-571.

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry

AU - Nakajima, Kazuki

AU - Kohyama-Koganeya, Ayako

AU - Hirabayashi, Yoshio

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Cell membrane surface glycosphingolipids (GSLs), which are synthesized from glucosylceramide (GlcCer) by the enzymatic action of glucosylceramide synthase (GlcT-1), play fundamental roles in many cellular processes. Recent studies have shown that in adipose tissue, GlcCer is involved in the regulation of energy (fat and sugar) metabolism and that GlcCer acts as a glucose donor in cholesteryl glucoside biosynthesis. To investigate the physiological functions of GlcT-1 and GlcCer, a method to profile GlcCer species by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been recently established. The method is based on a combination analysis of retention times in hydrophilic liquid chromatography (HILIC) with specified head group survey, such as neutral-loss and/or precursor-ion scans. Here, the application of this method for profiling GlcCer species in Drosophila melanogaster fat bodies in which Drosophila GLCT-1 (dGlcT-1) expression was manipulated is described, and the implications of these studies in the context of energy metabolism and obesity are discussed.

AB - Cell membrane surface glycosphingolipids (GSLs), which are synthesized from glucosylceramide (GlcCer) by the enzymatic action of glucosylceramide synthase (GlcT-1), play fundamental roles in many cellular processes. Recent studies have shown that in adipose tissue, GlcCer is involved in the regulation of energy (fat and sugar) metabolism and that GlcCer acts as a glucose donor in cholesteryl glucoside biosynthesis. To investigate the physiological functions of GlcT-1 and GlcCer, a method to profile GlcCer species by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been recently established. The method is based on a combination analysis of retention times in hydrophilic liquid chromatography (HILIC) with specified head group survey, such as neutral-loss and/or precursor-ion scans. Here, the application of this method for profiling GlcCer species in Drosophila melanogaster fat bodies in which Drosophila GLCT-1 (dGlcT-1) expression was manipulated is described, and the implications of these studies in the context of energy metabolism and obesity are discussed.

UR - http://www.scopus.com/inward/record.url?scp=84943627316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943627316&partnerID=8YFLogxK

U2 - 10.1007/978-4-431-54841-6_123

DO - 10.1007/978-4-431-54841-6_123

M3 - Chapter

AN - SCOPUS:84943627316

SN - 9784431548409

SP - 565

EP - 571

BT - Glycoscience

PB - Springer Japan

ER -

Nakajima K, Kohyama-Koganeya A, Hirabayashi Y. Profiling of glucosylceramides by liquid chromatography-tandem mass spectrometry. In Glycoscience: Biology and Medicine. Springer Japan. 2015. p. 565-571 https://doi.org/10.1007/978-4-431-54841-6_123