Profiling of serum extracellular vesicles reveals miRNA-4525 as a potential biomarker for advanced renal cell carcinoma

Yuka Muramatsu-Maekawa; Kyojiro Kawakami; Yasunori Fujita; Manabu Takai; Daiki Kato; Keita Nakane; Taku Kato; Tomohiro Tsuchiya; Takuya Koie; Yuri Miura; Masafumi Ito; Kosuke Mizutani

doi:10.21873/CGP.20256

Profiling of serum extracellular vesicles reveals miRNA-4525 as a potential biomarker for advanced renal cell carcinoma

Yuka Muramatsu-Maekawa, Kyojiro Kawakami, Yasunori Fujita, Manabu Takai, Daiki Kato, Keita Nakane, Taku Kato, Tomohiro Tsuchiya, Takuya Koie, Yuri Miura, Masafumi Ito, Kosuke Mizutani

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC.

Original language	English
Pages (from-to)	253-259
Number of pages	7
Journal	Cancer Genomics and Proteomics
Volume	18
Issue number	3
DOIs	https://doi.org/10.21873/CGP.20256
Publication status	Published - 05-2021
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Genetics
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{ebc7046c0c63485eae66ae578fe87425,

title = "Profiling of serum extracellular vesicles reveals miRNA-4525 as a potential biomarker for advanced renal cell carcinoma",

abstract = "Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC.",

keywords = "Extracellular vesicle, Microarray analysis, Mirna-4525, Proteomic analysis, Renal cell carcinoma",

author = "Yuka Muramatsu-Maekawa and Kyojiro Kawakami and Yasunori Fujita and Manabu Takai and Daiki Kato and Keita Nakane and Taku Kato and Tomohiro Tsuchiya and Takuya Koie and Yuri Miura and Masafumi Ito and Kosuke Mizutani",

year = "2021",

month = may,

doi = "10.21873/CGP.20256",

language = "English",

volume = "18",

pages = "253--259",

journal = "Cancer Genomics and Proteomics",

issn = "1109-6535",

publisher = "International Institute of Anticancer Research",

number = "3",

}

TY - JOUR

T1 - Profiling of serum extracellular vesicles reveals miRNA-4525 as a potential biomarker for advanced renal cell carcinoma

AU - Muramatsu-Maekawa, Yuka

AU - Kawakami, Kyojiro

AU - Fujita, Yasunori

AU - Takai, Manabu

AU - Kato, Daiki

AU - Nakane, Keita

AU - Kato, Taku

AU - Tsuchiya, Tomohiro

AU - Koie, Takuya

AU - Miura, Yuri

AU - Ito, Masafumi

AU - Mizutani, Kosuke

PY - 2021/5

Y1 - 2021/5

N2 - Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC.

AB - Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs). Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets. Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets. Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC.

KW - Extracellular vesicle

KW - Microarray analysis

KW - Mirna-4525

KW - Proteomic analysis

KW - Renal cell carcinoma

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U2 - 10.21873/CGP.20256

DO - 10.21873/CGP.20256

M3 - Article

C2 - 33893078

AN - SCOPUS:85105338997

SN - 1109-6535

VL - 18

SP - 253

EP - 259

JO - Cancer Genomics and Proteomics

JF - Cancer Genomics and Proteomics

IS - 3

ER -

Profiling of serum extracellular vesicles reveals miRNA-4525 as a potential biomarker for advanced renal cell carcinoma

Abstract

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