Prognostic value of proliferating cell nuclear antigen expression in chronic lymphoid leukemia

Auro Del Giglio, Susan O'Brien, Richard Ford, Hideyuki Saya, John Manning, Michael Keating, Dennis Johnston, Rainer Khetan, Adel El-Naggar, Albert Deisseroth

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Chronic lymphocytic leukemia (CLL) is a usually indolent disease that can assume an aggressive clinical course in some patients. To develop assays that would be predictive of how a particular patient's disease would evolve, we studied the expression of proliferating cell nuclear antigen (PCNA) by Western blotting in 40 patients with CLL. The concentration of PCNA, a cofactor for δDNA-dependent DNA polymerase, is indicative of the proliferative state of the cell. Significantly lower PCNA levels were observed in earlier stage CLL when compared with more advanced disease. The leukemic cell proliferative rate, assessed by lymphocyte doubling time and flow cytometry, also correlated significantly with the level of PCNA expression. These results suggest that a high level of PCNA in the cells of CLL patients at presentation identifies a subgroup of patients whose CLL cells have a higher proliferative activity and who may, therefore, have a potentially shorter survival.

Original languageEnglish
Pages (from-to)2717-2720
Number of pages4
Issue number10
Publication statusPublished - 15-05-1992

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology


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