TY - JOUR
T1 - Prominent Role for Plasmacytoid Dendritic Cells in Mucosal T Cell-Independent IgA Induction
AU - Tezuka, Hiroyuki
AU - Abe, Yukiko
AU - Asano, Jumpei
AU - Sato, Taku
AU - Liu, Jiajia
AU - Iwata, Makoto
AU - Ohteki, Toshiaki
N1 - Funding Information:
We thank H. Kamioka for secretarial support, H. Ishikawa (Keio University) for the B6.Tcrb −/− Tcrd −/− mice, T. Seya (Hokkaido University) for the B6.Ifnb −/− mice, and S. Akira and M. Yamamoto (Osaka University) for the B6.Myd88 −/− Trif −/− mice. This work was supported by a Grant-in-Aid for Young Scientists (B) (H.T.), the Takeda Science Foundation (T.O.), a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan (T.O.), and Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST) (T.O.).
PY - 2011/2/25
Y1 - 2011/2/25
N2 - Although both conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) are present in the gut-associated lymphoid tissues (GALT), the roles of pDCs in the gut remain largely unknown. Here we show a critical role for pDCs in T cell-independent (TI) IgA production by B cells in the GALT. When pDCs of the mesenteric lymph nodes (MLNs) and Peyer's patches (PPs) (which are representative GALT) were cultured with naive B cells to induce TI IgA class switch recombination (CSR), IgA production was substantially higher than in cocultures of these cells with cDCs. IgA production was dependent on APRIL and BAFF production by pDCs. Importantly, pDC expression of APRIL and BAFF was dependent on stromal cell-derived type I IFN signaling under steady-state conditions. Our findings provide insight into the molecular basis of pDC conditioning to induce mucosal TI IgA production, which may lead to improvements in vaccination strategies and treatment for mucosal-related disorders.
AB - Although both conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) are present in the gut-associated lymphoid tissues (GALT), the roles of pDCs in the gut remain largely unknown. Here we show a critical role for pDCs in T cell-independent (TI) IgA production by B cells in the GALT. When pDCs of the mesenteric lymph nodes (MLNs) and Peyer's patches (PPs) (which are representative GALT) were cultured with naive B cells to induce TI IgA class switch recombination (CSR), IgA production was substantially higher than in cocultures of these cells with cDCs. IgA production was dependent on APRIL and BAFF production by pDCs. Importantly, pDC expression of APRIL and BAFF was dependent on stromal cell-derived type I IFN signaling under steady-state conditions. Our findings provide insight into the molecular basis of pDC conditioning to induce mucosal TI IgA production, which may lead to improvements in vaccination strategies and treatment for mucosal-related disorders.
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U2 - 10.1016/j.immuni.2011.02.002
DO - 10.1016/j.immuni.2011.02.002
M3 - Article
C2 - 21333555
AN - SCOPUS:79951729700
SN - 1074-7613
VL - 34
SP - 247
EP - 257
JO - Immunity
JF - Immunity
IS - 2
ER -
