TY - JOUR
T1 - Properties of a New Picorna-Like Virus of the Brown-Winged Green Bug, Plautia stali
AU - Nakashima, Nobuhiko
AU - Sasaki, Jun
AU - Tsuda, Katuo
AU - Yasunaga, Chisa
AU - Noda, Hiroaki
N1 - Funding Information:
This work was supported by Special Coordination Funds for Promoting Science and Technology, Science and Technology Agency, Japan.
PY - 1998/3
Y1 - 1998/3
N2 - A picorna-like virus was isolated from the brown-winged green bug,Plautia stali.The virus was named Plautia stali intestine virus (PSIV) based on the multiplication site of the virus in the infected insects. PSIV is a spherical particle with a diameter of 30 nm. Particles of PSIV were found to contain a 9.1-kb single-stranded RNA. Polyacrylamide gel electrophoresis of purified PSIV particles revealed three major (33, 30, 26 kDa), one medium (35 kDa), and one minor (4.5 kDa) structural proteins. The molar ratios of the proteins suggested that the 35-, 33-, 30-, 26-, and 4.5-kDa proteins corresponded to VP0, VP1, VP2, VP3, and VP4 of vertebrate picornaviruses. Immunological assays indicated that PSIV and Nezara viridula virus-1, which is a picorna-like virus of the green stinkbug in South Africa, were serologically distinct. PSIV was detected in the intestine with enzyme-linked immunosorbent assay but not in the salivary glands, fat bodies, Malpighian tubules, and reproductive organs of viruliferousP. stali.The virus was also detected on the surface of the eggs and in the feces of infected insects. These results suggest that excrement of infected insects are the primary inoculum of the virus in a colony ofP. stali.The nonviruliferous adults ofP. staliusually survive a few months in laboratory, while the average of adulthood lifetime in viruliferousP. staliwas about 13 days. PSIV also infected two other stinkbugs,Nezara viridulaandHalyomorpha halys.
AB - A picorna-like virus was isolated from the brown-winged green bug,Plautia stali.The virus was named Plautia stali intestine virus (PSIV) based on the multiplication site of the virus in the infected insects. PSIV is a spherical particle with a diameter of 30 nm. Particles of PSIV were found to contain a 9.1-kb single-stranded RNA. Polyacrylamide gel electrophoresis of purified PSIV particles revealed three major (33, 30, 26 kDa), one medium (35 kDa), and one minor (4.5 kDa) structural proteins. The molar ratios of the proteins suggested that the 35-, 33-, 30-, 26-, and 4.5-kDa proteins corresponded to VP0, VP1, VP2, VP3, and VP4 of vertebrate picornaviruses. Immunological assays indicated that PSIV and Nezara viridula virus-1, which is a picorna-like virus of the green stinkbug in South Africa, were serologically distinct. PSIV was detected in the intestine with enzyme-linked immunosorbent assay but not in the salivary glands, fat bodies, Malpighian tubules, and reproductive organs of viruliferousP. stali.The virus was also detected on the surface of the eggs and in the feces of infected insects. These results suggest that excrement of infected insects are the primary inoculum of the virus in a colony ofP. stali.The nonviruliferous adults ofP. staliusually survive a few months in laboratory, while the average of adulthood lifetime in viruliferousP. staliwas about 13 days. PSIV also infected two other stinkbugs,Nezara viridulaandHalyomorpha halys.
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U2 - 10.1006/jipa.1997.4721
DO - 10.1006/jipa.1997.4721
M3 - Article
AN - SCOPUS:0000780364
SN - 0022-2011
VL - 71
SP - 151
EP - 158
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 2
ER -