Properties of a New Picorna-Like Virus of the Brown-Winged Green Bug, Plautia stali

Nobuhiko Nakashima, Jun Sasaki, Katuo Tsuda, Chisa Yasunaga, Hiroaki Noda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A picorna-like virus was isolated from the brown-winged green bug,Plautia stali.The virus was named Plautia stali intestine virus (PSIV) based on the multiplication site of the virus in the infected insects. PSIV is a spherical particle with a diameter of 30 nm. Particles of PSIV were found to contain a 9.1-kb single-stranded RNA. Polyacrylamide gel electrophoresis of purified PSIV particles revealed three major (33, 30, 26 kDa), one medium (35 kDa), and one minor (4.5 kDa) structural proteins. The molar ratios of the proteins suggested that the 35-, 33-, 30-, 26-, and 4.5-kDa proteins corresponded to VP0, VP1, VP2, VP3, and VP4 of vertebrate picornaviruses. Immunological assays indicated that PSIV and Nezara viridula virus-1, which is a picorna-like virus of the green stinkbug in South Africa, were serologically distinct. PSIV was detected in the intestine with enzyme-linked immunosorbent assay but not in the salivary glands, fat bodies, Malpighian tubules, and reproductive organs of viruliferousP. stali.The virus was also detected on the surface of the eggs and in the feces of infected insects. These results suggest that excrement of infected insects are the primary inoculum of the virus in a colony ofP. stali.The nonviruliferous adults ofP. staliusually survive a few months in laboratory, while the average of adulthood lifetime in viruliferousP. staliwas about 13 days. PSIV also infected two other stinkbugs,Nezara viridulaandHalyomorpha halys.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalJournal of Invertebrate Pathology
Volume71
Issue number2
DOIs
Publication statusPublished - 01-03-1998

Fingerprint

Plautia stali intestine virus
Plautia stali
virus
viruses
Nezara viridula virus-1
Nezara
insect viruses
Picornaviridae
insects
Malpighian tubules
eggs
insect
structural proteins
excreta
salivary glands
bug
adulthood
immunoassays
virion
protein

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

Cite this

Nakashima, Nobuhiko ; Sasaki, Jun ; Tsuda, Katuo ; Yasunaga, Chisa ; Noda, Hiroaki. / Properties of a New Picorna-Like Virus of the Brown-Winged Green Bug, Plautia stali. In: Journal of Invertebrate Pathology. 1998 ; Vol. 71, No. 2. pp. 151-158.
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Properties of a New Picorna-Like Virus of the Brown-Winged Green Bug, Plautia stali. / Nakashima, Nobuhiko; Sasaki, Jun; Tsuda, Katuo; Yasunaga, Chisa; Noda, Hiroaki.

In: Journal of Invertebrate Pathology, Vol. 71, No. 2, 01.03.1998, p. 151-158.

Research output: Contribution to journalArticle

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