Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells

Hanayuki Okura, Hiroshi Komoda, Ayami Saga, Aya Kakuta-Yamamoto, Yoko Hamada, Yuichi Fumimoto, Chun Man Lee, Akihiro Ichinose, Yoshiki Sawa, Akifumi Matsuyama

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

There are only a few reports that describe the hepatocytic differentiation potential of human adipose tissue-derived mesenchymal stem cells (hADMSCs) and no reports that describe the in vivo functions of hepatocyte-like cells differentiated from somatic stem cells including hADMSCs. In this study, we established a new method for generation of functional hepatocyte-like cell clusters using floating culture method and induced functional hepatocyte-like cell clusters, which functioned effectively not only in vitro but also in vivo. The generated hepatocyte-like cell clusters were characterized by gene expression analysis, functional assays, and transplantation into non-obese diabetic severe combined immunodeficiency (NOD-SCID) mouse with chronic liver injury. The generated hepatocyte-like cell clusters expressed various genes normally found on mature hepatocytes. The cell clusters exhibited functional characteristics of hepatocytes: they expressed albumin, secreted urea, had cytochrome P450 activity, could take up low-density lipoprotein, and stored glycogen. Transplantation of these cell clusters into NOD-SCID mouse with chronic liver injury resulted in a significant improvement of serum albumin and total bilirubin levels. In summary, we established a new protocol for efficient induction of hADMSCs into functional hepatocyte-like cell clusters.

Original languageEnglish
Pages (from-to)761-770
Number of pages10
JournalTissue Engineering - Part C: Methods
Volume16
Issue number4
DOIs
Publication statusPublished - 01-08-2010
Externally publishedYes

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Adipose Tissue
Hepatocytes
Tissue
Severe Combined Immunodeficiency
Liver
Lipoproteins
Functional analysis
Adult Stem Cells
Cell Transplantation
Wounds and Injuries
Glycogen
LDL Lipoproteins
Bilirubin
Gene expression
Urea
Serum Albumin
Cytochrome P-450 Enzyme System
Albumins

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering

Cite this

Okura, Hanayuki ; Komoda, Hiroshi ; Saga, Ayami ; Kakuta-Yamamoto, Aya ; Hamada, Yoko ; Fumimoto, Yuichi ; Lee, Chun Man ; Ichinose, Akihiro ; Sawa, Yoshiki ; Matsuyama, Akifumi. / Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells. In: Tissue Engineering - Part C: Methods. 2010 ; Vol. 16, No. 4. pp. 761-770.
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Okura, H, Komoda, H, Saga, A, Kakuta-Yamamoto, A, Hamada, Y, Fumimoto, Y, Lee, CM, Ichinose, A, Sawa, Y & Matsuyama, A 2010, 'Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells', Tissue Engineering - Part C: Methods, vol. 16, no. 4, pp. 761-770. https://doi.org/10.1089/ten.tec.2009.0208

Properties of hepatocyte-like cell clusters from human adipose tissue-derived mesenchymal stem cells. / Okura, Hanayuki; Komoda, Hiroshi; Saga, Ayami; Kakuta-Yamamoto, Aya; Hamada, Yoko; Fumimoto, Yuichi; Lee, Chun Man; Ichinose, Akihiro; Sawa, Yoshiki; Matsuyama, Akifumi.

In: Tissue Engineering - Part C: Methods, Vol. 16, No. 4, 01.08.2010, p. 761-770.

Research output: Contribution to journalArticle

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AU - Hamada, Yoko

AU - Fumimoto, Yuichi

AU - Lee, Chun Man

AU - Ichinose, Akihiro

AU - Sawa, Yoshiki

AU - Matsuyama, Akifumi

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Y1 - 2010/8/1

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