TY - JOUR
T1 - Propolis induces Ca2+ signaling in immune cells
AU - Adachi, Takahiro
AU - Yoshikawa, Soichiro
AU - Tezuka, Hiroyuki
AU - Tsuji, Noriko M.
AU - Ohteki, Toshiaki
AU - Karasuyama, Hajime
AU - Kumazawa, Toshihiko
N1 - Funding Information:
We are grateful to Dr. K. Rajewsky (MDC) for the CD19-Cre and IgG1-Cre mice, Dr. M. Okabe (Osaka University) for the CAG-Cre mice, Yamada bee company, Inc. for propolis and Drs. K. Hashimoto and H. Tani (Yamada Bee Company, Inc.) for critical reading of the manuscript. This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Yamada Foundation (to T.A.), the Joint Usage/Research Program of the Medical Research Institute, Tokyo Medical and Dental University (to S.Y., H.K.. and N.M.T.), a Grant-in-Aid from the Cross-ministerial Strategic Innovation Promotion Program (SIP) (to N.M.T.), and the Canon Foundation (to T.A. and N.M.T.).
Funding Information:
We are grateful to Dr. K. Rajewsky (MDC) for the CD19-Cre and IgG1-Cre mice, Dr. M. Okabe (Osaka University) for the CAG-Cre mice, Yamada bee company, Inc. for propolis and Drs. K. Hashimoto and H. Tani (Yamada Bee Company, Inc.) for critical reading of the manuscript. This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Yamada Foundation (to T.A.), the Joint Usage/Research Program of the Medical Research Institute, Tokyo Medical and Dental University (to S.Y., H.K. and N.M.T.), a Grant-in-Aid from the Cross-ministerial Strategic Innovation Promotion Program (SIP) (to N.M.T.), and the Canon Foundation (to T.A. and N.M.T.).
Publisher Copyright:
© 2019 BMFH Press.
PY - 2019
Y1 - 2019
N2 - Propolis possesses several immunological functions. We recently generated a conditional Ca2+ biosensor yellow cameleon (YC3.60) transgenic mouse line and established a five-dimensional (5D) (x, y, z, time, and Ca2+ signaling) system for intravital imaging of lymphoid tissues, including Peyer's patches (PPs). To assess the effects of propolis on immune cells, we analyzed Ca2+ signaling in vitro and in vivo using CD11c-Cre/YC3.60flox transgenic mice, in which CD11c+ dendritic cells (DCs) specifically express YC3.60. We found that propolis induced Ca2+ signaling in DCs in the PPs. Intravital imaging of PPs also showed that an intraperitoneal injection of propolis augmented Ca2+ signaling in CD11c+ cells, suggesting that propolis possesses immune-stimulating activity. Furthermore, CD11c+ cells in PPs in mice administrated propolis indicated an increase in Ca2+ signaling. Our results indicate that propolis induces immunogenicity under physiological conditions.
AB - Propolis possesses several immunological functions. We recently generated a conditional Ca2+ biosensor yellow cameleon (YC3.60) transgenic mouse line and established a five-dimensional (5D) (x, y, z, time, and Ca2+ signaling) system for intravital imaging of lymphoid tissues, including Peyer's patches (PPs). To assess the effects of propolis on immune cells, we analyzed Ca2+ signaling in vitro and in vivo using CD11c-Cre/YC3.60flox transgenic mice, in which CD11c+ dendritic cells (DCs) specifically express YC3.60. We found that propolis induced Ca2+ signaling in DCs in the PPs. Intravital imaging of PPs also showed that an intraperitoneal injection of propolis augmented Ca2+ signaling in CD11c+ cells, suggesting that propolis possesses immune-stimulating activity. Furthermore, CD11c+ cells in PPs in mice administrated propolis indicated an increase in Ca2+ signaling. Our results indicate that propolis induces immunogenicity under physiological conditions.
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U2 - 10.12938/bmfh.19-011
DO - 10.12938/bmfh.19-011
M3 - Article
AN - SCOPUS:85074363092
VL - 38
SP - 141
EP - 149
JO - Bioscience of Microbiota, Food and Health
JF - Bioscience of Microbiota, Food and Health
SN - 2186-6953
IS - 4
ER -