TY - JOUR
T1 - Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
AU - Morikawa, Satoru
AU - Mabuchi, Yo
AU - Kubota, Yoshiaki
AU - Nagai, Yasuo
AU - Niibe, Kunimichi
AU - Hiratsu, Emi
AU - Suzuki, Sadafumi
AU - Miyauchi-Hara, Chikako
AU - Nagoshi, Narihito
AU - Sunabori, Takehiko
AU - Shimmura, Shigeto
AU - Miyawaki, Atsushi
AU - Nakagawa, Taneaki
AU - Suda, Toshio
AU - Okano, Hideyuki
AU - Matsuzaki, Yumi
PY - 2009/10/26
Y1 - 2009/10/26
N2 - Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRα+Sca-1+CD45 -TER119-) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.
AB - Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRα+Sca-1+CD45 -TER119-) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.
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U2 - 10.1084/jem.20091046
DO - 10.1084/jem.20091046
M3 - Article
C2 - 19841085
AN - SCOPUS:70449701931
SN - 0022-1007
VL - 206
SP - 2483
EP - 2496
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 11
ER -