Abstract
We investigated the effect of prostaglandin E1 (PGE1) on the secretion of interleukin-6 (IL-6) in osteoblast-like MC3T3-E1 cells. PGE1, which induced cAMP accumulation, stimulated IL-6 secretion time dependently up to 48 h. The stimulative effect of PGE1 was dose-dependent in the range between 10 nM and 10 μM. Cholera toxin, an activator of G(s), stimulated IL-6 secretion in MC3T3-E1 cells. Forskolin, which directly activates adenylate cyclase, significantly induced IL-6 secretion in a dose-dependent manner in the range between 1 and 50 μM. Dibutyryl cAMP (Bt2cAMP) stimulated IL-6 secretion time-dependently up to 48 h. The effect of Bt2cAMP on IL-6 secretion was dose-dependent in the range between 0.1 and 3 mM. N-[2-(p-bromocin-namylamino)ethyl]-5-isoquinoline-sulfonamide (H-89), a potent and selective inhibitor of protein kinase A, which suppressed the IL-6 secretion induced by forskolin or Bt2cAMP, significantly inhibited the IL-6 secretion induced by PGE1. These results indicate that PGE1 stimulates IL-6 secretion via the activation of protein kinase A in osteoblast-like cells.
| Original language | English |
|---|---|
| Pages (from-to) | 105-108 |
| Number of pages | 4 |
| Journal | Cellular Signalling |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 01-01-1997 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Cell Biology
Cite this
}
Prostaglandin E1 stimulates interleukin-6 secretion via protein kinase A in osteoblast-like cells. / Watanabe-Tomita, Yasuko; Suzuki, Atsushi; Oiso, Yutaka; Kozawa, Osamu.
In: Cellular Signalling, Vol. 9, No. 1, 01.01.1997, p. 105-108.Research output: Contribution to journal › Article
TY - JOUR
T1 - Prostaglandin E1 stimulates interleukin-6 secretion via protein kinase A in osteoblast-like cells
AU - Watanabe-Tomita, Yasuko
AU - Suzuki, Atsushi
AU - Oiso, Yutaka
AU - Kozawa, Osamu
PY - 1997/1/1
Y1 - 1997/1/1
N2 - We investigated the effect of prostaglandin E1 (PGE1) on the secretion of interleukin-6 (IL-6) in osteoblast-like MC3T3-E1 cells. PGE1, which induced cAMP accumulation, stimulated IL-6 secretion time dependently up to 48 h. The stimulative effect of PGE1 was dose-dependent in the range between 10 nM and 10 μM. Cholera toxin, an activator of G(s), stimulated IL-6 secretion in MC3T3-E1 cells. Forskolin, which directly activates adenylate cyclase, significantly induced IL-6 secretion in a dose-dependent manner in the range between 1 and 50 μM. Dibutyryl cAMP (Bt2cAMP) stimulated IL-6 secretion time-dependently up to 48 h. The effect of Bt2cAMP on IL-6 secretion was dose-dependent in the range between 0.1 and 3 mM. N-[2-(p-bromocin-namylamino)ethyl]-5-isoquinoline-sulfonamide (H-89), a potent and selective inhibitor of protein kinase A, which suppressed the IL-6 secretion induced by forskolin or Bt2cAMP, significantly inhibited the IL-6 secretion induced by PGE1. These results indicate that PGE1 stimulates IL-6 secretion via the activation of protein kinase A in osteoblast-like cells.
AB - We investigated the effect of prostaglandin E1 (PGE1) on the secretion of interleukin-6 (IL-6) in osteoblast-like MC3T3-E1 cells. PGE1, which induced cAMP accumulation, stimulated IL-6 secretion time dependently up to 48 h. The stimulative effect of PGE1 was dose-dependent in the range between 10 nM and 10 μM. Cholera toxin, an activator of G(s), stimulated IL-6 secretion in MC3T3-E1 cells. Forskolin, which directly activates adenylate cyclase, significantly induced IL-6 secretion in a dose-dependent manner in the range between 1 and 50 μM. Dibutyryl cAMP (Bt2cAMP) stimulated IL-6 secretion time-dependently up to 48 h. The effect of Bt2cAMP on IL-6 secretion was dose-dependent in the range between 0.1 and 3 mM. N-[2-(p-bromocin-namylamino)ethyl]-5-isoquinoline-sulfonamide (H-89), a potent and selective inhibitor of protein kinase A, which suppressed the IL-6 secretion induced by forskolin or Bt2cAMP, significantly inhibited the IL-6 secretion induced by PGE1. These results indicate that PGE1 stimulates IL-6 secretion via the activation of protein kinase A in osteoblast-like cells.
UR - http://www.scopus.com/inward/record.url?scp=0030615106&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030615106&partnerID=8YFLogxK
U2 - 10.1016/S0898-6568(96)00114-3
DO - 10.1016/S0898-6568(96)00114-3
M3 - Article
C2 - 9067638
AN - SCOPUS:0030615106
VL - 9
SP - 105
EP - 108
JO - Cellular Signalling
JF - Cellular Signalling
SN - 0898-6568
IS - 1
ER -