Prostaglandin F activates phospholipase D independently from activation of protein kinase C in osteoblast‐like cells

Osamu Kozawa, Atsushi Suzuki, Jun Kotoyori, Haruhiko Tokuda, Yasuko Watanabe, Yoshiaki Ito, Yutaka Oiso

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55 Citations (Scopus)

Abstract

We previously reported that prostaglandin F (PGF) receptor is coupled to pertussis toxin (PTX)–sensitive GTP‐binding protein (G protein) in osteoblast‐like MC3T3‐E1 cells [Miwa et al. (1990): Biochem Biophys Res Commun 171:1229–1235]. In the present study, we examined the effect of PGF on the activation of phosphatidylcholine‐hydrolyzing phospholipase D in MC3T3‐E1 cells. PGF stimulated the formation of choline in a dose‐dependent manner in the range between 10 nM and 10 μM. The formation of choline was stimulated by 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a protein kinase C (PKC)–activating phorbol ester. 4α‐Phorbol 12,13‐didecanoate, a PKC‐nonactivating phorbol ester, had little effect on choline formation. The formation of choline stimulated by a combination of PGF and TPA was additive. Staurosporine, an inhibitor for protein kinases, which inhibited the effect of TPA on choline formation, dose‐dependently enhanced the formation of choline induced by PGF. NaF, an activator of G protein, stimulated the formation of choline. The formation of choline stimulated by a combination of PGF and NaF was not additive. NaF‐induced formation of choline was dose‐dependently enhanced by staurosporine. PTX dose‐dependently inhibited the PGF‐induced formation of choline. These results strongly suggest that PGF activates phospholipase D independently from the activation of PKC in osteoblast‐like cells and PTX‐sensitive G protein is involved in the PGF‐induced phospholipase D activation. © 1994 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)373-379
Number of pages7
JournalJournal of Cellular Biochemistry
Volume55
Issue number3
DOIs
Publication statusPublished - 07-1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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