Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

Takao Tsuji, Takeshi Shimizu, Keiko Sasaki, Yoshiyasu Shimizu, Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Satoshi Sugiyama, Koki Taniguchi, Paola Neri, Hiroshi Mori

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalVaccine
Volume26
Issue number4
DOIs
Publication statusPublished - 24-01-2008

Fingerprint

Shiga Toxin 2
toxemia
Shiga-like toxin 2
Shiga Toxin
Toxemia
Shiga toxin
Enterotoxins
enterotoxins
Nose
Vaccines
vaccines
Escherichia coli
intranasal administration
Intranasal Administration
cholera toxin
antibodies
Escherichia coli O157
Cholera Toxin
mice
Neutralizing Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Tsuji, Takao ; Shimizu, Takeshi ; Sasaki, Keiko ; Shimizu, Yoshiyasu ; Tsukamoto, Kentaro ; Arimitsu, Hideyuki ; Ochi, Sadayuki ; Sugiyama, Satoshi ; Taniguchi, Koki ; Neri, Paola ; Mori, Hiroshi. / Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin. In: Vaccine. 2008 ; Vol. 26, No. 4. pp. 469-476.
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Tsuji, T, Shimizu, T, Sasaki, K, Shimizu, Y, Tsukamoto, K, Arimitsu, H, Ochi, S, Sugiyama, S, Taniguchi, K, Neri, P & Mori, H 2008, 'Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin', Vaccine, vol. 26, no. 4, pp. 469-476. https://doi.org/10.1016/j.vaccine.2007.11.038

Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin. / Tsuji, Takao; Shimizu, Takeshi; Sasaki, Keiko; Shimizu, Yoshiyasu; Tsukamoto, Kentaro; Arimitsu, Hideyuki; Ochi, Sadayuki; Sugiyama, Satoshi; Taniguchi, Koki; Neri, Paola; Mori, Hiroshi.

In: Vaccine, Vol. 26, No. 4, 24.01.2008, p. 469-476.

Research output: Contribution to journalArticle

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T1 - Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

AU - Tsuji, Takao

AU - Shimizu, Takeshi

AU - Sasaki, Keiko

AU - Shimizu, Yoshiyasu

AU - Tsukamoto, Kentaro

AU - Arimitsu, Hideyuki

AU - Ochi, Sadayuki

AU - Sugiyama, Satoshi

AU - Taniguchi, Koki

AU - Neri, Paola

AU - Mori, Hiroshi

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AB - Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

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