Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

Takao Tsuji; Takeshi Shimizu; Keiko Sasaki; Yoshiyasu Shimizu; Kentaro Tsukamoto; Hideyuki Arimitsu; Sadayuki Ochi; Satoshi Sugiyama; Koki Taniguchi; Paola Neri; Hiroshi Mori

doi:10.1016/j.vaccine.2007.11.038

Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

Takao Tsuji, Takeshi Shimizu, Keiko Sasaki, Yoshiyasu Shimizu, Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Satoshi Sugiyama, Koki Taniguchi, Paola Neri, Hiroshi Mori

Microbiology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

Original language	English
Pages (from-to)	469-476
Number of pages	8
Journal	Vaccine
Volume	26
Issue number	4
DOIs	https://doi.org/10.1016/j.vaccine.2007.11.038
Publication status	Published - 24-01-2008

All Science Journal Classification (ASJC) codes

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2007.11.038

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title = "Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin",

abstract = "Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.",

author = "Takao Tsuji and Takeshi Shimizu and Keiko Sasaki and Yoshiyasu Shimizu and Kentaro Tsukamoto and Hideyuki Arimitsu and Sadayuki Ochi and Satoshi Sugiyama and Koki Taniguchi and Paola Neri and Hiroshi Mori",

note = "Funding Information: This work was supported by a grants-in-aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology and Japan Society for the Promotion of Science (JSPS) and for the Open Research Center's Project of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2008",

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doi = "10.1016/j.vaccine.2007.11.038",

language = "English",

volume = "26",

pages = "469--476",

journal = "Vaccine",

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T1 - Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

AU - Tsuji, Takao

AU - Shimizu, Takeshi

AU - Sasaki, Keiko

AU - Shimizu, Yoshiyasu

AU - Tsukamoto, Kentaro

AU - Arimitsu, Hideyuki

AU - Ochi, Sadayuki

AU - Sugiyama, Satoshi

AU - Taniguchi, Koki

AU - Neri, Paola

AU - Mori, Hiroshi

N1 - Funding Information: This work was supported by a grants-in-aid for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology and Japan Society for the Promotion of Science (JSPS) and for the Open Research Center's Project of Fujita Health University from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2008/1/24

Y1 - 2008/1/24

N2 - Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

AB - Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.

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SN - 0264-410X

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SP - 469

EP - 476

JO - Vaccine

JF - Vaccine

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Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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