Protective effect of botulinum C/D mosaic toxoid against avian botulism

Masato Takeda, Hiroko Kasai, Yasushi Torii, Masafumi Mukamoto, Tomoko Kohda, Kentaro Tsukamoto, Shunji Kozaki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Avian botulism is a paralytic disease caused by a toxin produced by Clostridium botulinum type C. Since type C isolates from cases of avian botulism produced a neurotoxin consisting of a mosaic form of parts of type C and D neurotoxins, we examined the antitoxin titers in the convalescent sera of botulism-affected birds which belonged to family Anatidae. ELISA using the C/D mosaic neurotoxin as an antigen revealed that the antibody was detected in the sera at 2 weeks, but not at 5 weeks after the onset, suggesting that the antibody only appeared for a short period in the convalescent phase. However, we failed to detect the antibody titers with anti-chicken IgG instead of anti-duck IgG. We therefore examine the immunological properties of IgG among different families and species. The results revealed that different species of IgG in the same family exhibited strong cross-reactivity. Ducks immunized once with the toxoid together with a commercial oil-adjuvanted vaccine were found to develop sufficient antibody to protect against a challenge with a lethal toxin dose. The ELISA titers did not correspond to the neutralization titers in the sera of immunized ducks at the early stage during immunization. These findings suggest that the neutralizing titer was more useful than the ELISA titer for evaluating the protection against the toxin, but the ELISA technique may be applicable for detecting the occurrence of botulism.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalJournal of Veterinary Medical Science
Volume68
Issue number4
DOIs
Publication statusPublished - 01-04-2006

Fingerprint

Toxoids
Botulism
botulism
toxoids
protective effect
neurotoxins
Ducks
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
ducks
antibodies
toxins
Antibodies
neutralization
Immunoglobulin G
Serum
Clostridium botulinum C
Anatidae
antitoxins
Antitoxins

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Takeda, Masato ; Kasai, Hiroko ; Torii, Yasushi ; Mukamoto, Masafumi ; Kohda, Tomoko ; Tsukamoto, Kentaro ; Kozaki, Shunji. / Protective effect of botulinum C/D mosaic toxoid against avian botulism. In: Journal of Veterinary Medical Science. 2006 ; Vol. 68, No. 4. pp. 325-330.
@article{379b2dc1a4144d9c8478731fe958adab,
title = "Protective effect of botulinum C/D mosaic toxoid against avian botulism",
abstract = "Avian botulism is a paralytic disease caused by a toxin produced by Clostridium botulinum type C. Since type C isolates from cases of avian botulism produced a neurotoxin consisting of a mosaic form of parts of type C and D neurotoxins, we examined the antitoxin titers in the convalescent sera of botulism-affected birds which belonged to family Anatidae. ELISA using the C/D mosaic neurotoxin as an antigen revealed that the antibody was detected in the sera at 2 weeks, but not at 5 weeks after the onset, suggesting that the antibody only appeared for a short period in the convalescent phase. However, we failed to detect the antibody titers with anti-chicken IgG instead of anti-duck IgG. We therefore examine the immunological properties of IgG among different families and species. The results revealed that different species of IgG in the same family exhibited strong cross-reactivity. Ducks immunized once with the toxoid together with a commercial oil-adjuvanted vaccine were found to develop sufficient antibody to protect against a challenge with a lethal toxin dose. The ELISA titers did not correspond to the neutralization titers in the sera of immunized ducks at the early stage during immunization. These findings suggest that the neutralizing titer was more useful than the ELISA titer for evaluating the protection against the toxin, but the ELISA technique may be applicable for detecting the occurrence of botulism.",
author = "Masato Takeda and Hiroko Kasai and Yasushi Torii and Masafumi Mukamoto and Tomoko Kohda and Kentaro Tsukamoto and Shunji Kozaki",
year = "2006",
month = "4",
day = "1",
doi = "10.1292/jvms.68.325",
language = "English",
volume = "68",
pages = "325--330",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "4",

}

Takeda, M, Kasai, H, Torii, Y, Mukamoto, M, Kohda, T, Tsukamoto, K & Kozaki, S 2006, 'Protective effect of botulinum C/D mosaic toxoid against avian botulism', Journal of Veterinary Medical Science, vol. 68, no. 4, pp. 325-330. https://doi.org/10.1292/jvms.68.325

Protective effect of botulinum C/D mosaic toxoid against avian botulism. / Takeda, Masato; Kasai, Hiroko; Torii, Yasushi; Mukamoto, Masafumi; Kohda, Tomoko; Tsukamoto, Kentaro; Kozaki, Shunji.

In: Journal of Veterinary Medical Science, Vol. 68, No. 4, 01.04.2006, p. 325-330.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect of botulinum C/D mosaic toxoid against avian botulism

AU - Takeda, Masato

AU - Kasai, Hiroko

AU - Torii, Yasushi

AU - Mukamoto, Masafumi

AU - Kohda, Tomoko

AU - Tsukamoto, Kentaro

AU - Kozaki, Shunji

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Avian botulism is a paralytic disease caused by a toxin produced by Clostridium botulinum type C. Since type C isolates from cases of avian botulism produced a neurotoxin consisting of a mosaic form of parts of type C and D neurotoxins, we examined the antitoxin titers in the convalescent sera of botulism-affected birds which belonged to family Anatidae. ELISA using the C/D mosaic neurotoxin as an antigen revealed that the antibody was detected in the sera at 2 weeks, but not at 5 weeks after the onset, suggesting that the antibody only appeared for a short period in the convalescent phase. However, we failed to detect the antibody titers with anti-chicken IgG instead of anti-duck IgG. We therefore examine the immunological properties of IgG among different families and species. The results revealed that different species of IgG in the same family exhibited strong cross-reactivity. Ducks immunized once with the toxoid together with a commercial oil-adjuvanted vaccine were found to develop sufficient antibody to protect against a challenge with a lethal toxin dose. The ELISA titers did not correspond to the neutralization titers in the sera of immunized ducks at the early stage during immunization. These findings suggest that the neutralizing titer was more useful than the ELISA titer for evaluating the protection against the toxin, but the ELISA technique may be applicable for detecting the occurrence of botulism.

AB - Avian botulism is a paralytic disease caused by a toxin produced by Clostridium botulinum type C. Since type C isolates from cases of avian botulism produced a neurotoxin consisting of a mosaic form of parts of type C and D neurotoxins, we examined the antitoxin titers in the convalescent sera of botulism-affected birds which belonged to family Anatidae. ELISA using the C/D mosaic neurotoxin as an antigen revealed that the antibody was detected in the sera at 2 weeks, but not at 5 weeks after the onset, suggesting that the antibody only appeared for a short period in the convalescent phase. However, we failed to detect the antibody titers with anti-chicken IgG instead of anti-duck IgG. We therefore examine the immunological properties of IgG among different families and species. The results revealed that different species of IgG in the same family exhibited strong cross-reactivity. Ducks immunized once with the toxoid together with a commercial oil-adjuvanted vaccine were found to develop sufficient antibody to protect against a challenge with a lethal toxin dose. The ELISA titers did not correspond to the neutralization titers in the sera of immunized ducks at the early stage during immunization. These findings suggest that the neutralizing titer was more useful than the ELISA titer for evaluating the protection against the toxin, but the ELISA technique may be applicable for detecting the occurrence of botulism.

UR - http://www.scopus.com/inward/record.url?scp=33646479308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646479308&partnerID=8YFLogxK

U2 - 10.1292/jvms.68.325

DO - 10.1292/jvms.68.325

M3 - Article

VL - 68

SP - 325

EP - 330

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 4

ER -