Protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress

Tadashi Nakamura, Yoshiji Ohta, Koji Ohashi, Kumiko Ikeno, Rie Watanabe, Kenji Tokunaga, Nobuhiro Harada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the present study we examined the protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress (WIRS) in comparison with that of vitamin E (VE). Fasted rats orally received Brazilian green propolis ethanol extract (BPEE; 10, 50 or 100 mg/kg), VE (250 mg/kg) or vehicle at 30 min before the onset of WIRS. Exposure of vehicle-treated rats to 6 h of WIRS caused liver cell damage, judging from the levels of serum alanine aminotransferase and aspartate aminotransferease, increased hepatic lipid peroxide, NOx contents and myeloperoxidase activity, and decreased hepatic non-protein SH, ascorbic acid contents and superoxide dismutase activity. Preadministration of BPEE (50 or 100 mg/kg) or VE to the stressed rats protected against the hepatic damage and attenuated the increased hepatic lipid peroxide and NOx contents and myeloperoxidase activity and the decreased hepatic non-protein SH and ascorbic acid contents and superoxide dismutase activity. These protective effects of BPEE (50 mg/kg) were greater than those of BPEE (100 mg/kg) and were almost equal to those of VE. These results indicate that BPEE protects against hepatic oxidative damage in rats exposed to WIRS possibly through its antioxidant and antiinflammatory properties such as VE.

Original languageEnglish
Pages (from-to)1482-1489
Number of pages8
JournalPhytotherapy Research
Volume26
Issue number10
DOIs
Publication statusPublished - 01-10-2012

Fingerprint

Propolis
Immersion
Vitamin E
Water
Liver
Lipid Peroxides
Peroxidase
Ascorbic Acid
Superoxide Dismutase
Selegiline
Alanine Transaminase
Aspartic Acid
Anti-Inflammatory Agents
Ethanol
Antioxidants

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Nakamura, Tadashi ; Ohta, Yoshiji ; Ohashi, Koji ; Ikeno, Kumiko ; Watanabe, Rie ; Tokunaga, Kenji ; Harada, Nobuhiro. / Protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress. In: Phytotherapy Research. 2012 ; Vol. 26, No. 10. pp. 1482-1489.
@article{51a57272af7b4945ab6cf6d63ab1fd83,
title = "Protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress",
abstract = "In the present study we examined the protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress (WIRS) in comparison with that of vitamin E (VE). Fasted rats orally received Brazilian green propolis ethanol extract (BPEE; 10, 50 or 100 mg/kg), VE (250 mg/kg) or vehicle at 30 min before the onset of WIRS. Exposure of vehicle-treated rats to 6 h of WIRS caused liver cell damage, judging from the levels of serum alanine aminotransferase and aspartate aminotransferease, increased hepatic lipid peroxide, NOx contents and myeloperoxidase activity, and decreased hepatic non-protein SH, ascorbic acid contents and superoxide dismutase activity. Preadministration of BPEE (50 or 100 mg/kg) or VE to the stressed rats protected against the hepatic damage and attenuated the increased hepatic lipid peroxide and NOx contents and myeloperoxidase activity and the decreased hepatic non-protein SH and ascorbic acid contents and superoxide dismutase activity. These protective effects of BPEE (50 mg/kg) were greater than those of BPEE (100 mg/kg) and were almost equal to those of VE. These results indicate that BPEE protects against hepatic oxidative damage in rats exposed to WIRS possibly through its antioxidant and antiinflammatory properties such as VE.",
author = "Tadashi Nakamura and Yoshiji Ohta and Koji Ohashi and Kumiko Ikeno and Rie Watanabe and Kenji Tokunaga and Nobuhiro Harada",
year = "2012",
month = "10",
day = "1",
doi = "10.1002/ptr.4601",
language = "English",
volume = "26",
pages = "1482--1489",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "10",

}

Protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress. / Nakamura, Tadashi; Ohta, Yoshiji; Ohashi, Koji; Ikeno, Kumiko; Watanabe, Rie; Tokunaga, Kenji; Harada, Nobuhiro.

In: Phytotherapy Research, Vol. 26, No. 10, 01.10.2012, p. 1482-1489.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress

AU - Nakamura, Tadashi

AU - Ohta, Yoshiji

AU - Ohashi, Koji

AU - Ikeno, Kumiko

AU - Watanabe, Rie

AU - Tokunaga, Kenji

AU - Harada, Nobuhiro

PY - 2012/10/1

Y1 - 2012/10/1

N2 - In the present study we examined the protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress (WIRS) in comparison with that of vitamin E (VE). Fasted rats orally received Brazilian green propolis ethanol extract (BPEE; 10, 50 or 100 mg/kg), VE (250 mg/kg) or vehicle at 30 min before the onset of WIRS. Exposure of vehicle-treated rats to 6 h of WIRS caused liver cell damage, judging from the levels of serum alanine aminotransferase and aspartate aminotransferease, increased hepatic lipid peroxide, NOx contents and myeloperoxidase activity, and decreased hepatic non-protein SH, ascorbic acid contents and superoxide dismutase activity. Preadministration of BPEE (50 or 100 mg/kg) or VE to the stressed rats protected against the hepatic damage and attenuated the increased hepatic lipid peroxide and NOx contents and myeloperoxidase activity and the decreased hepatic non-protein SH and ascorbic acid contents and superoxide dismutase activity. These protective effects of BPEE (50 mg/kg) were greater than those of BPEE (100 mg/kg) and were almost equal to those of VE. These results indicate that BPEE protects against hepatic oxidative damage in rats exposed to WIRS possibly through its antioxidant and antiinflammatory properties such as VE.

AB - In the present study we examined the protective effect of Brazilian propolis against hepatic oxidative damage in rats with water-immersion restraint stress (WIRS) in comparison with that of vitamin E (VE). Fasted rats orally received Brazilian green propolis ethanol extract (BPEE; 10, 50 or 100 mg/kg), VE (250 mg/kg) or vehicle at 30 min before the onset of WIRS. Exposure of vehicle-treated rats to 6 h of WIRS caused liver cell damage, judging from the levels of serum alanine aminotransferase and aspartate aminotransferease, increased hepatic lipid peroxide, NOx contents and myeloperoxidase activity, and decreased hepatic non-protein SH, ascorbic acid contents and superoxide dismutase activity. Preadministration of BPEE (50 or 100 mg/kg) or VE to the stressed rats protected against the hepatic damage and attenuated the increased hepatic lipid peroxide and NOx contents and myeloperoxidase activity and the decreased hepatic non-protein SH and ascorbic acid contents and superoxide dismutase activity. These protective effects of BPEE (50 mg/kg) were greater than those of BPEE (100 mg/kg) and were almost equal to those of VE. These results indicate that BPEE protects against hepatic oxidative damage in rats exposed to WIRS possibly through its antioxidant and antiinflammatory properties such as VE.

UR - http://www.scopus.com/inward/record.url?scp=84867336397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867336397&partnerID=8YFLogxK

U2 - 10.1002/ptr.4601

DO - 10.1002/ptr.4601

M3 - Article

C2 - 22298415

AN - SCOPUS:84867336397

VL - 26

SP - 1482

EP - 1489

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 10

ER -