Abstract
We examined the neuroprotective role of nicergoline in neuron-microglia or neuron-astrocytes co-cultures. Nicergoline, an ergoline derivative, significantly suppressed the neuronal cell death induced by co-culture with activated microglia or astrocytes stimulated with lipopolysaccharide (LPS) and interferon (IFN)-γ. To elucidate the mechanism by which nicergoline exerts a neuroprotective effect, we examined the production of inflammatory mediators and neurotrophic factors in activated microglia and astrocytes following nicergoline treatment. In microglia stimulated with LPS and IFN-γ, nicergoline suppressed the production of superoxide anions, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in a dose-dependent manner. In astrocytes, nicergoline also suppressed the production of proinflammatory cytokines and enhanced brain-derived neurotrophic factor (BDNF). Thus, nicergoline-mediated neuroprotection resulted primarily from the inhibition of inflammatory mediators and the upregulation of neurotrophic factors by glial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 78-85 |
| Number of pages | 8 |
| Journal | Brain Research |
| Volume | 1066 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 20-12-2005 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
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Protective effects of nicergoline against neuronal cell death induced by activated microglia and astrocytes. / Mizuno, Tetsuya; Kuno, Reiko; Nitta, Atsumi; Nabeshima, Toshitaka; Zhang, Guiqin; Kawanokuchi, Jun; Wang, Jinyan; Jin, Shijie; Takeuchi, Hideyuki; Suzumura, Akio.
In: Brain Research, Vol. 1066, No. 1-2, 20.12.2005, p. 78-85.Research output: Contribution to journal › Article
TY - JOUR
T1 - Protective effects of nicergoline against neuronal cell death induced by activated microglia and astrocytes
AU - Mizuno, Tetsuya
AU - Kuno, Reiko
AU - Nitta, Atsumi
AU - Nabeshima, Toshitaka
AU - Zhang, Guiqin
AU - Kawanokuchi, Jun
AU - Wang, Jinyan
AU - Jin, Shijie
AU - Takeuchi, Hideyuki
AU - Suzumura, Akio
PY - 2005/12/20
Y1 - 2005/12/20
N2 - We examined the neuroprotective role of nicergoline in neuron-microglia or neuron-astrocytes co-cultures. Nicergoline, an ergoline derivative, significantly suppressed the neuronal cell death induced by co-culture with activated microglia or astrocytes stimulated with lipopolysaccharide (LPS) and interferon (IFN)-γ. To elucidate the mechanism by which nicergoline exerts a neuroprotective effect, we examined the production of inflammatory mediators and neurotrophic factors in activated microglia and astrocytes following nicergoline treatment. In microglia stimulated with LPS and IFN-γ, nicergoline suppressed the production of superoxide anions, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in a dose-dependent manner. In astrocytes, nicergoline also suppressed the production of proinflammatory cytokines and enhanced brain-derived neurotrophic factor (BDNF). Thus, nicergoline-mediated neuroprotection resulted primarily from the inhibition of inflammatory mediators and the upregulation of neurotrophic factors by glial cells.
AB - We examined the neuroprotective role of nicergoline in neuron-microglia or neuron-astrocytes co-cultures. Nicergoline, an ergoline derivative, significantly suppressed the neuronal cell death induced by co-culture with activated microglia or astrocytes stimulated with lipopolysaccharide (LPS) and interferon (IFN)-γ. To elucidate the mechanism by which nicergoline exerts a neuroprotective effect, we examined the production of inflammatory mediators and neurotrophic factors in activated microglia and astrocytes following nicergoline treatment. In microglia stimulated with LPS and IFN-γ, nicergoline suppressed the production of superoxide anions, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in a dose-dependent manner. In astrocytes, nicergoline also suppressed the production of proinflammatory cytokines and enhanced brain-derived neurotrophic factor (BDNF). Thus, nicergoline-mediated neuroprotection resulted primarily from the inhibition of inflammatory mediators and the upregulation of neurotrophic factors by glial cells.
UR - http://www.scopus.com/inward/record.url?scp=29044446068&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29044446068&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2005.10.050
DO - 10.1016/j.brainres.2005.10.050
M3 - Article
C2 - 16325157
AN - SCOPUS:29044446068
VL - 1066
SP - 78
EP - 85
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -