Protective role of genetic polymorphism of heat shock protein 70-2 for gastric cancer risk

Tomoyuki Shibata, Tomiyasu Arisawa, Tomomitsu Tahara, Daisuke Yoshioka, Naoko Maruyama, Hiroshi Fujita, Yoshio Kamiya, Masakatsu Nakamura, Mitsuo Nagasaka, Masami Iwata, Kazuya Takahama, Makoto Watanabe, Ichiro Hirata, Hiroshi Nakano

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Abstract

Background Heat shock protein 70-2 (HSP70-2) has a cytoprotective role in various conditions and also protects the gastric mucosa. Recently, polymorphism of HSP70-2 at position 1267 was suggested to be associated with carcinogenesis. We investigated the association of this polymorphism with the risk of gastric cancer in the present study. Methods We examined 223 patients (159 men and 64 women, mean age 64.8 years) with gastric cancer who underwent gastrointestinal endoscopy at our department. The controls were 200 age-matched patients (140 men and 60 women) without gastric cancer diagnosed by gastrointestinal endoscopy. Genotyping was done by PCR-based restriction fragment length polymorphism analysis, and the PCR products were digested with PstI. The two allelic forms, corresponding to the presence or absence of the PstI site, were designated as the P1 allele and P2 allele, respectively. Logistic regression analysis was performed to calculate an odds ratios (ORs) for differences of HSP70-2 polymorphism between the two groups. Results Among the 223 patients with gastric cancer, 46 (20.6%) had P1/P1, 177 (79.4%) were P1 carriers, and 6 (2.7%) were P2/P2. In the control group, 33 (16.5%) patients had P1/P1 polymorphism, 167 (83.5%) were P1 carriers, and 12 (6.0%) were P2/P2. The OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.43 (95% CI = 0.16-1.17) (P = 0.097). Among females, the OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.10 (95% CI = 0.012-0.838) (P = 0.014). This polymorphism was also associated with a lower risk of middle third cancer (OR = 0.13; 95% CI = 0.02-1.00). Conclusions P2/P2 polymorphism of HSP70-2 at position 1267 was associated with a lower risk of gastric cancer in females.

Original languageEnglish
Pages (from-to)70-74
Number of pages5
JournalDigestive Diseases and Sciences
Volume54
Issue number1
DOIs
Publication statusPublished - 01-01-2009

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HSP70 Heat-Shock Proteins
Genetic Polymorphisms
Stomach Neoplasms
Odds Ratio
Gastrointestinal Endoscopy
Alleles
Polymerase Chain Reaction
Gastric Mucosa
Restriction Fragment Length Polymorphisms
Carcinogenesis
Logistic Models
Regression Analysis
Control Groups

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Shibata, Tomoyuki ; Arisawa, Tomiyasu ; Tahara, Tomomitsu ; Yoshioka, Daisuke ; Maruyama, Naoko ; Fujita, Hiroshi ; Kamiya, Yoshio ; Nakamura, Masakatsu ; Nagasaka, Mitsuo ; Iwata, Masami ; Takahama, Kazuya ; Watanabe, Makoto ; Hirata, Ichiro ; Nakano, Hiroshi. / Protective role of genetic polymorphism of heat shock protein 70-2 for gastric cancer risk. In: Digestive Diseases and Sciences. 2009 ; Vol. 54, No. 1. pp. 70-74.
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abstract = "Background Heat shock protein 70-2 (HSP70-2) has a cytoprotective role in various conditions and also protects the gastric mucosa. Recently, polymorphism of HSP70-2 at position 1267 was suggested to be associated with carcinogenesis. We investigated the association of this polymorphism with the risk of gastric cancer in the present study. Methods We examined 223 patients (159 men and 64 women, mean age 64.8 years) with gastric cancer who underwent gastrointestinal endoscopy at our department. The controls were 200 age-matched patients (140 men and 60 women) without gastric cancer diagnosed by gastrointestinal endoscopy. Genotyping was done by PCR-based restriction fragment length polymorphism analysis, and the PCR products were digested with PstI. The two allelic forms, corresponding to the presence or absence of the PstI site, were designated as the P1 allele and P2 allele, respectively. Logistic regression analysis was performed to calculate an odds ratios (ORs) for differences of HSP70-2 polymorphism between the two groups. Results Among the 223 patients with gastric cancer, 46 (20.6{\%}) had P1/P1, 177 (79.4{\%}) were P1 carriers, and 6 (2.7{\%}) were P2/P2. In the control group, 33 (16.5{\%}) patients had P1/P1 polymorphism, 167 (83.5{\%}) were P1 carriers, and 12 (6.0{\%}) were P2/P2. The OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.43 (95{\%} CI = 0.16-1.17) (P = 0.097). Among females, the OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.10 (95{\%} CI = 0.012-0.838) (P = 0.014). This polymorphism was also associated with a lower risk of middle third cancer (OR = 0.13; 95{\%} CI = 0.02-1.00). Conclusions P2/P2 polymorphism of HSP70-2 at position 1267 was associated with a lower risk of gastric cancer in females.",
author = "Tomoyuki Shibata and Tomiyasu Arisawa and Tomomitsu Tahara and Daisuke Yoshioka and Naoko Maruyama and Hiroshi Fujita and Yoshio Kamiya and Masakatsu Nakamura and Mitsuo Nagasaka and Masami Iwata and Kazuya Takahama and Makoto Watanabe and Ichiro Hirata and Hiroshi Nakano",
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Shibata, T, Arisawa, T, Tahara, T, Yoshioka, D, Maruyama, N, Fujita, H, Kamiya, Y, Nakamura, M, Nagasaka, M, Iwata, M, Takahama, K, Watanabe, M, Hirata, I & Nakano, H 2009, 'Protective role of genetic polymorphism of heat shock protein 70-2 for gastric cancer risk', Digestive Diseases and Sciences, vol. 54, no. 1, pp. 70-74. https://doi.org/10.1007/s10620-008-0313-z

Protective role of genetic polymorphism of heat shock protein 70-2 for gastric cancer risk. / Shibata, Tomoyuki; Arisawa, Tomiyasu; Tahara, Tomomitsu; Yoshioka, Daisuke; Maruyama, Naoko; Fujita, Hiroshi; Kamiya, Yoshio; Nakamura, Masakatsu; Nagasaka, Mitsuo; Iwata, Masami; Takahama, Kazuya; Watanabe, Makoto; Hirata, Ichiro; Nakano, Hiroshi.

In: Digestive Diseases and Sciences, Vol. 54, No. 1, 01.01.2009, p. 70-74.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective role of genetic polymorphism of heat shock protein 70-2 for gastric cancer risk

AU - Shibata, Tomoyuki

AU - Arisawa, Tomiyasu

AU - Tahara, Tomomitsu

AU - Yoshioka, Daisuke

AU - Maruyama, Naoko

AU - Fujita, Hiroshi

AU - Kamiya, Yoshio

AU - Nakamura, Masakatsu

AU - Nagasaka, Mitsuo

AU - Iwata, Masami

AU - Takahama, Kazuya

AU - Watanabe, Makoto

AU - Hirata, Ichiro

AU - Nakano, Hiroshi

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Background Heat shock protein 70-2 (HSP70-2) has a cytoprotective role in various conditions and also protects the gastric mucosa. Recently, polymorphism of HSP70-2 at position 1267 was suggested to be associated with carcinogenesis. We investigated the association of this polymorphism with the risk of gastric cancer in the present study. Methods We examined 223 patients (159 men and 64 women, mean age 64.8 years) with gastric cancer who underwent gastrointestinal endoscopy at our department. The controls were 200 age-matched patients (140 men and 60 women) without gastric cancer diagnosed by gastrointestinal endoscopy. Genotyping was done by PCR-based restriction fragment length polymorphism analysis, and the PCR products were digested with PstI. The two allelic forms, corresponding to the presence or absence of the PstI site, were designated as the P1 allele and P2 allele, respectively. Logistic regression analysis was performed to calculate an odds ratios (ORs) for differences of HSP70-2 polymorphism between the two groups. Results Among the 223 patients with gastric cancer, 46 (20.6%) had P1/P1, 177 (79.4%) were P1 carriers, and 6 (2.7%) were P2/P2. In the control group, 33 (16.5%) patients had P1/P1 polymorphism, 167 (83.5%) were P1 carriers, and 12 (6.0%) were P2/P2. The OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.43 (95% CI = 0.16-1.17) (P = 0.097). Among females, the OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.10 (95% CI = 0.012-0.838) (P = 0.014). This polymorphism was also associated with a lower risk of middle third cancer (OR = 0.13; 95% CI = 0.02-1.00). Conclusions P2/P2 polymorphism of HSP70-2 at position 1267 was associated with a lower risk of gastric cancer in females.

AB - Background Heat shock protein 70-2 (HSP70-2) has a cytoprotective role in various conditions and also protects the gastric mucosa. Recently, polymorphism of HSP70-2 at position 1267 was suggested to be associated with carcinogenesis. We investigated the association of this polymorphism with the risk of gastric cancer in the present study. Methods We examined 223 patients (159 men and 64 women, mean age 64.8 years) with gastric cancer who underwent gastrointestinal endoscopy at our department. The controls were 200 age-matched patients (140 men and 60 women) without gastric cancer diagnosed by gastrointestinal endoscopy. Genotyping was done by PCR-based restriction fragment length polymorphism analysis, and the PCR products were digested with PstI. The two allelic forms, corresponding to the presence or absence of the PstI site, were designated as the P1 allele and P2 allele, respectively. Logistic regression analysis was performed to calculate an odds ratios (ORs) for differences of HSP70-2 polymorphism between the two groups. Results Among the 223 patients with gastric cancer, 46 (20.6%) had P1/P1, 177 (79.4%) were P1 carriers, and 6 (2.7%) were P2/P2. In the control group, 33 (16.5%) patients had P1/P1 polymorphism, 167 (83.5%) were P1 carriers, and 12 (6.0%) were P2/P2. The OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.43 (95% CI = 0.16-1.17) (P = 0.097). Among females, the OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.10 (95% CI = 0.012-0.838) (P = 0.014). This polymorphism was also associated with a lower risk of middle third cancer (OR = 0.13; 95% CI = 0.02-1.00). Conclusions P2/P2 polymorphism of HSP70-2 at position 1267 was associated with a lower risk of gastric cancer in females.

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