Protein kinase C activation by interleukin (IL)-1 limits IL-1-induced IL-6 synthesis in osteoblast-like cells: Involvement of phosphatidylcholine- specific phospholipase C

Osamu Kozawa, Atsushi Suzuki, Haruhiko Tokuda, Takehiro Kaida, Toshihiko Uematsu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We investigated the regulatory mechanism of interleukin-6 (IL-6) synthesis induced by interleukin-1 (IL-1) in osteoblast-like MC3T3-E1 cells. IL-1 stimulated the secretion of IL-6 in a dose-dependent manner in the range between 0.1 and 100 ng/ml. Staurosporine and calphostin C, inhibitors of protein kinase C (PKC), significantly enhanced the IL-1-induced secretion of IL-6. The stimulative effect of IL-1 was markedly amplified in PKC down- regulated MC3T3-E1 cells. IL-1 produced diacylglycerol in MC3T3-E1 cells. IL- 1 had little effect on the formation of inositol phosphates and choline. On the contrary, IL-1 significantly stimulated the formation of phosphocholine dose-dependently. D-609, an inhibitor of phosphatidylcholine-specific phospholipase C, suppressed the IL-1-induced diacylglycerol production. The IL-1 -induced IL-6 secretion was significantly enhanced by D-609. These results indicate that IL-1 activates PKC via phosphatidylcholine-specific phospholipase C in osteoblast-like cells, and the PKC activation then limits IL-6 synthesis induced by IL-1 itself.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalJournal of Cellular Biochemistry
Volume67
Issue number1
DOIs
Publication statusPublished - 01-10-1997

Fingerprint

Osteoblasts
Interleukin-1
Protein Kinase C
Interleukin-6
Chemical activation
Diglycerides
phosphatidylcholine-specific phospholipase C
Inositol Phosphates
Staurosporine
Phosphorylcholine
Choline

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{1a308289aae94fe798c1d2a580f3af83,
title = "Protein kinase C activation by interleukin (IL)-1 limits IL-1-induced IL-6 synthesis in osteoblast-like cells: Involvement of phosphatidylcholine- specific phospholipase C",
abstract = "We investigated the regulatory mechanism of interleukin-6 (IL-6) synthesis induced by interleukin-1 (IL-1) in osteoblast-like MC3T3-E1 cells. IL-1 stimulated the secretion of IL-6 in a dose-dependent manner in the range between 0.1 and 100 ng/ml. Staurosporine and calphostin C, inhibitors of protein kinase C (PKC), significantly enhanced the IL-1-induced secretion of IL-6. The stimulative effect of IL-1 was markedly amplified in PKC down- regulated MC3T3-E1 cells. IL-1 produced diacylglycerol in MC3T3-E1 cells. IL- 1 had little effect on the formation of inositol phosphates and choline. On the contrary, IL-1 significantly stimulated the formation of phosphocholine dose-dependently. D-609, an inhibitor of phosphatidylcholine-specific phospholipase C, suppressed the IL-1-induced diacylglycerol production. The IL-1 -induced IL-6 secretion was significantly enhanced by D-609. These results indicate that IL-1 activates PKC via phosphatidylcholine-specific phospholipase C in osteoblast-like cells, and the PKC activation then limits IL-6 synthesis induced by IL-1 itself.",
author = "Osamu Kozawa and Atsushi Suzuki and Haruhiko Tokuda and Takehiro Kaida and Toshihiko Uematsu",
year = "1997",
month = "10",
day = "1",
doi = "10.1002/(SICI)1097-4644(19971001)67:1<103::AID-JCB11>3.0.CO;2-I",
language = "English",
volume = "67",
pages = "103--111",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "1",

}

Protein kinase C activation by interleukin (IL)-1 limits IL-1-induced IL-6 synthesis in osteoblast-like cells : Involvement of phosphatidylcholine- specific phospholipase C. / Kozawa, Osamu; Suzuki, Atsushi; Tokuda, Haruhiko; Kaida, Takehiro; Uematsu, Toshihiko.

In: Journal of Cellular Biochemistry, Vol. 67, No. 1, 01.10.1997, p. 103-111.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein kinase C activation by interleukin (IL)-1 limits IL-1-induced IL-6 synthesis in osteoblast-like cells

T2 - Involvement of phosphatidylcholine- specific phospholipase C

AU - Kozawa, Osamu

AU - Suzuki, Atsushi

AU - Tokuda, Haruhiko

AU - Kaida, Takehiro

AU - Uematsu, Toshihiko

PY - 1997/10/1

Y1 - 1997/10/1

N2 - We investigated the regulatory mechanism of interleukin-6 (IL-6) synthesis induced by interleukin-1 (IL-1) in osteoblast-like MC3T3-E1 cells. IL-1 stimulated the secretion of IL-6 in a dose-dependent manner in the range between 0.1 and 100 ng/ml. Staurosporine and calphostin C, inhibitors of protein kinase C (PKC), significantly enhanced the IL-1-induced secretion of IL-6. The stimulative effect of IL-1 was markedly amplified in PKC down- regulated MC3T3-E1 cells. IL-1 produced diacylglycerol in MC3T3-E1 cells. IL- 1 had little effect on the formation of inositol phosphates and choline. On the contrary, IL-1 significantly stimulated the formation of phosphocholine dose-dependently. D-609, an inhibitor of phosphatidylcholine-specific phospholipase C, suppressed the IL-1-induced diacylglycerol production. The IL-1 -induced IL-6 secretion was significantly enhanced by D-609. These results indicate that IL-1 activates PKC via phosphatidylcholine-specific phospholipase C in osteoblast-like cells, and the PKC activation then limits IL-6 synthesis induced by IL-1 itself.

AB - We investigated the regulatory mechanism of interleukin-6 (IL-6) synthesis induced by interleukin-1 (IL-1) in osteoblast-like MC3T3-E1 cells. IL-1 stimulated the secretion of IL-6 in a dose-dependent manner in the range between 0.1 and 100 ng/ml. Staurosporine and calphostin C, inhibitors of protein kinase C (PKC), significantly enhanced the IL-1-induced secretion of IL-6. The stimulative effect of IL-1 was markedly amplified in PKC down- regulated MC3T3-E1 cells. IL-1 produced diacylglycerol in MC3T3-E1 cells. IL- 1 had little effect on the formation of inositol phosphates and choline. On the contrary, IL-1 significantly stimulated the formation of phosphocholine dose-dependently. D-609, an inhibitor of phosphatidylcholine-specific phospholipase C, suppressed the IL-1-induced diacylglycerol production. The IL-1 -induced IL-6 secretion was significantly enhanced by D-609. These results indicate that IL-1 activates PKC via phosphatidylcholine-specific phospholipase C in osteoblast-like cells, and the PKC activation then limits IL-6 synthesis induced by IL-1 itself.

UR - http://www.scopus.com/inward/record.url?scp=0030984057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030984057&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4644(19971001)67:1<103::AID-JCB11>3.0.CO;2-I

DO - 10.1002/(SICI)1097-4644(19971001)67:1<103::AID-JCB11>3.0.CO;2-I

M3 - Article

C2 - 9328844

AN - SCOPUS:0030984057

VL - 67

SP - 103

EP - 111

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 1

ER -