Protein kinase C and calcium ion in mitogenic response of macrophage-depleted human peripheral lymphocytes

K. Kaibuchi, Y. Takai, Y. Nishizuka

Research output: Contribution to journalArticlepeer-review

140 Citations (Scopus)

Abstract

For mitogenic response of macrophage-depleted human peripheral lymphocytes, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or 1-oleoyl-2-acetylglycerol (OAG) and Ca2+ ionophore are both needed in addition to a small quantity of plant lectin, phytohemagglutinin (PHA). PHA alone is not sufficient to produce the cellular response. The addition of TPA or OAG to these cells induces the activation of protein kinase C as assayed by the phosphorylation of its endogenous substrates. Apparently, TPA or OAG and A23187 together substitute for macrophages and act synergistically to potentiate the DNA synthesis of this lymphocyte preparation. The results suggest that protein kinase C activation and Ca2+ mobilization are essential and that additional receptor occupation by PHA is necessary for producing cell proliferation.

Original languageEnglish
Pages (from-to)1366-1369
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number3
Publication statusPublished - 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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