Protein SUMOylation is massively increased in hibernation torpor and is critical for the cytoprotection provided by ischemic preconditioning and hypothermia in SHSY5Y cells

Yang Ja Lee, Shin Ichi Miyake, Hideaki Wakita, David C. McMullen, Yoshiaki Azuma, Sungyoung Auh, John M. Hallenbeck

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Hibernation torpor provides an excellent natural model of tolerance to profound reductions in blood flow to the brain and other organs. Here, we report that during torpor of 13-lined ground squirrels, massive SUMOylation occurs in the brain, liver, and kidney. The level of small ubiquitin-related modifier (SUMO) conjugation coincides with the expression level of Ubc9, the SUMO specific E2-conjugating enzyme. Hypothermia alone also increased SUMO conjugation, but not as markedly as hibernation torpor. Increased SUMO conjugation (induced by Ubc9 overexpression, ischemic preconditioning (PC)±hypothermia) was necessary and sufficient for tolerance of SHSY5Y neuroblastoma cells to oxygen/glucose deprivation (OGD) ('in vitro ischemia'); decreased SUMO conjugation (induced by a dominant-negative Ubc9) severely reduced tolerance to OGD in these cells. These data indicate that post-translational modification of proteins by SUMOylation is a prominent feature of hibernation torpor and is critical for cytoprotection by ischemic PC±hypothermia in SHSY5Y cells subjected to OGD.

Original languageEnglish
Pages (from-to)950-962
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume27
Issue number5
DOIs
Publication statusPublished - 16-05-2007

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Torpor
Sumoylation
Hibernation
Ischemic Preconditioning
Cytoprotection
Ubiquitin
Hypothermia
Oxygen
Proteins
Glucose
Sciuridae
Brain
Post Translational Protein Processing
Neuroblastoma
Ischemia
Kidney
Liver
Enzymes

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Protein SUMOylation is massively increased in hibernation torpor and is critical for the cytoprotection provided by ischemic preconditioning and hypothermia in SHSY5Y cells",
abstract = "Hibernation torpor provides an excellent natural model of tolerance to profound reductions in blood flow to the brain and other organs. Here, we report that during torpor of 13-lined ground squirrels, massive SUMOylation occurs in the brain, liver, and kidney. The level of small ubiquitin-related modifier (SUMO) conjugation coincides with the expression level of Ubc9, the SUMO specific E2-conjugating enzyme. Hypothermia alone also increased SUMO conjugation, but not as markedly as hibernation torpor. Increased SUMO conjugation (induced by Ubc9 overexpression, ischemic preconditioning (PC)±hypothermia) was necessary and sufficient for tolerance of SHSY5Y neuroblastoma cells to oxygen/glucose deprivation (OGD) ('in vitro ischemia'); decreased SUMO conjugation (induced by a dominant-negative Ubc9) severely reduced tolerance to OGD in these cells. These data indicate that post-translational modification of proteins by SUMOylation is a prominent feature of hibernation torpor and is critical for cytoprotection by ischemic PC±hypothermia in SHSY5Y cells subjected to OGD.",
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Protein SUMOylation is massively increased in hibernation torpor and is critical for the cytoprotection provided by ischemic preconditioning and hypothermia in SHSY5Y cells. / Lee, Yang Ja; Miyake, Shin Ichi; Wakita, Hideaki; McMullen, David C.; Azuma, Yoshiaki; Auh, Sungyoung; Hallenbeck, John M.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 27, No. 5, 16.05.2007, p. 950-962.

Research output: Contribution to journalArticle

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AU - Lee, Yang Ja

AU - Miyake, Shin Ichi

AU - Wakita, Hideaki

AU - McMullen, David C.

AU - Azuma, Yoshiaki

AU - Auh, Sungyoung

AU - Hallenbeck, John M.

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N2 - Hibernation torpor provides an excellent natural model of tolerance to profound reductions in blood flow to the brain and other organs. Here, we report that during torpor of 13-lined ground squirrels, massive SUMOylation occurs in the brain, liver, and kidney. The level of small ubiquitin-related modifier (SUMO) conjugation coincides with the expression level of Ubc9, the SUMO specific E2-conjugating enzyme. Hypothermia alone also increased SUMO conjugation, but not as markedly as hibernation torpor. Increased SUMO conjugation (induced by Ubc9 overexpression, ischemic preconditioning (PC)±hypothermia) was necessary and sufficient for tolerance of SHSY5Y neuroblastoma cells to oxygen/glucose deprivation (OGD) ('in vitro ischemia'); decreased SUMO conjugation (induced by a dominant-negative Ubc9) severely reduced tolerance to OGD in these cells. These data indicate that post-translational modification of proteins by SUMOylation is a prominent feature of hibernation torpor and is critical for cytoprotection by ischemic PC±hypothermia in SHSY5Y cells subjected to OGD.

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