TY - JOUR
T1 - Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain
AU - Ota, Haruko
AU - Hikita, Takao
AU - Nishioka, Tomoki
AU - Matsumoto, Mami
AU - Ito, Jun
AU - Asai, Naoya
AU - Enomoto, Atsushi
AU - Takahashi, Masahide
AU - Kaibuchi, Kozo
AU - Sobue, Kazuya
AU - Sawamoto, Kazunobu
N1 - Funding Information:
This work was supported by the Funding Program for the Next Generation World-Leading Researchers (Japan Society for the Promotion of Science) and JSPS KAKENHI Grant Number 23700385 . Liquid chromatography tandem mass spectrometry (LC–MS/MS) shotgun analyses were supported by a Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network) from the Ministry of Education, Science, Sports and Culture of Japan .
PY - 2013/12/6
Y1 - 2013/12/6
N2 - Neural stem cells continuously generate new neurons in the ventricular-subventricular zone (V-SVZ) of the postnatal and adult mammalian brain. New neurons born in the rodent V-SVZ migrate toward the olfactory bulb (OB), where they differentiate into interneurons. To reveal novel intracellular molecular mechanisms that control postnatal neuronal migration, we performed a global proteomic search for proteins interacting with Girdin, an essential protein for postnatal neuronal migration. Using GST pull-down and LC-MS/MS shotgun analysis, we identified cytoskeletal proteins, cytoskeleton-binding proteins, and signal-transduction proteins as possible participants in neuronal migration. Our results suggest that Girdin and Girdin-interacting proteins control neuronal migration by regulating actin and/or microtubule dynamics.
AB - Neural stem cells continuously generate new neurons in the ventricular-subventricular zone (V-SVZ) of the postnatal and adult mammalian brain. New neurons born in the rodent V-SVZ migrate toward the olfactory bulb (OB), where they differentiate into interneurons. To reveal novel intracellular molecular mechanisms that control postnatal neuronal migration, we performed a global proteomic search for proteins interacting with Girdin, an essential protein for postnatal neuronal migration. Using GST pull-down and LC-MS/MS shotgun analysis, we identified cytoskeletal proteins, cytoskeleton-binding proteins, and signal-transduction proteins as possible participants in neuronal migration. Our results suggest that Girdin and Girdin-interacting proteins control neuronal migration by regulating actin and/or microtubule dynamics.
UR - https://www.scopus.com/pages/publications/84889650787
UR - https://www.scopus.com/pages/publications/84889650787#tab=citedBy
U2 - 10.1016/j.bbrc.2013.10.126
DO - 10.1016/j.bbrc.2013.10.126
M3 - Article
C2 - 24211587
AN - SCOPUS:84889650787
SN - 0006-291X
VL - 442
SP - 16
EP - 21
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1-2
ER -